Table 1 Specific forward and reverse primer sequences used in semi-quantitative RT-PCR
Gene | Primer forward (5′-3′) | Primer reverse (5′-3′) | Accession number |
|---|---|---|---|
NR (nitrate reductase) | 5′-TTCATGAACACTACCGACGTCG-3′ | 5′-GAGCCTGTACGGATACTCGGC-3′ | M27821 |
AS (asparagine synthetase) | 5′-CATCATTGAGCTCTCGCGCAGGTTAC-3′ | 5′-GGGGGAAATGTTATGAAGCGTTCACAA-3′ | X82849 |
CS (citrate synthetase) | 5′-GTTTGGTCATGGAGTTCTGCGTAA-3′ | 5′-GGAGGTACAACTTCATACAACTTGGACAC-3′ | W49861 |
IDH (isocitrate dehydrogenase) | 5′-AAACTCGAGGCTGCTTGCGTTGAGA-3′ | 5′-ATAATTAGCTTGCATCGAAACTGCGG-3′ | W21690 |
MDH (malate dehydrogenase) | 5′-GCCAGATTTCTGAGAGACTTAATGTCCA-3′ | 5′-TCGAGGCATGAGTAAGCAAGCGTCTT-3′ | T27564 |
18S | 5′-CCATCCCTCCGTAGTTAGCTTCT-3′ | 5′-CCTGTCGGCCAAGGCTATATAC-3′ | U42796.1 |