Table 1 Gradient elution programs of spermidine
Time(min) | Flow rate(mL min−1) | Percentage of mobile phase (%) | |
|---|---|---|---|
Methanol (Mobile phase A) | Water (Mobile phase B) | ||
0. 00 | 0.8 | 80 | 20 |
3.00 | 0.8 | 80 | 20 |
14.00 | 0.8 | 100 | 0 |
16.00 | 0.8 | 100 | 0 |
27.00 | 0.8 | 80 | 20 |
40.00 | 0.8 | 80 | 20 |