Table 2 Gradient elution programs of paeoniflorin
Time (min) | Flow rate (mL min−1) | Percentage of mobile phase (%) | |
|---|---|---|---|
Acetonitrile (Mobile phase A) | 0.015% Phosphoric acid water (Mobile phase B) | ||
0. 00 | 0.8 | 8 | 92 |
5.00 | 0.8 | 12 | 88 |
17.50 | 0.8 | 13 | 87 |
20.00 | 0.8 | 14 | 86 |
38.00 | 0.8 | 20 | 80 |
48.00 | 0.8 | 30 | 70 |
63.00 | 0.8 | 60 | 40 |
73.00 | 0.8 | 8 | 92 |