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Chemical and Biological Technologies in Agriculture

Table 1 Experimental design with bacterial inoculants in combination with enzymes in straw silage

From: Bacterial inoculants and enzymes based silage cocktails boost the ensiling quality of biomasses from reed, corn and rice straw

Biomass

Cocktaila

Straw (g)

Molasses (mL)

Water (mL)

Bacterial inoculants (CFU)

Enzymes (U)

L. plantarum

L. buchneri

P. pentosaceus

A. niger

B. subtilis

C. utilis

xylanase

β-Mannanases

glucanase

Reed straw

R-Mesa

500

20

500

1.0 × 106

1.4 × 107

3.0 × 105

8.0 × 108

1.6 × 106

1.0 × 109

5.0 × 104

2.5 × 103

1.0 × 104

R-MesaA

500

20

500

1.0 × 106

1.4 × 107

3.0 × 105

1.6 × 109

1.6 × 106

1.0 × 109

5.0 × 104

2.5 × 103

1.0 × 104

R-MesaB

500

20

500

1.0 × 106

1.4 × 107

3.0 × 105

8.0 × 108

3.2 × 106

1.0 × 109

5.0 × 104

2.5 × 103

1.0 × 104

R-MesaC

500

20

500

1.0 × 106

1.4 × 107

3.0 × 105

8.0 × 108

1.6 × 106

2.0 × 109

5.0 × 104

2.5 × 103

1.0 × 104

R-MesaG

500

20

500

1.0 × 106

1.4 × 107

3.0 × 105

8.0 × 108

1.6 × 106

1.0 × 109

5.0 × 104

2.5 × 103

2.0 × 104

R-Control

500

/

500

/

/

/

/

/

/

/

/

–

Corn straw

C-Mesa

500

20

710

1.0 × 106

1.4 × 107

3.0 × 105

4.0 × 108

8.0 × 105

5.0 × 108

5.0 × 104

2.5 × 103

1.0 × 104

C-MesaA

500

20

710

1.0 × 106

1.4 × 107

3.0 × 105

1.2 × 109

8.0 × 105

5.0 × 108

5.0 × 104

2.5 × 103

1.0 × 104

C-MesaB

500

20

710

1.0 × 106

1.4 × 107

3.0 × 105

4.0 × 108

2.4 × 106

5.0 × 108

5.0 × 104

2.5 × 103

1.0 × 104

C-MesaC

500

20

710

1.0 × 106

1.4 × 107

3.0 × 105

4.0 × 108

8.0 × 105

1.5 × 109

5.0 × 104

2.5 × 103

1.0 × 104

C-MesaG

500

20

710

1.0 × 106

1.4 × 107

3.0 × 105

4.0 × 108

8.0 × 105

5.0 × 108

5.0 × 104

2.5 × 103

2.0 × 104

C-Control

500

/

710

/

/

/

/

/

/

/

/

–

Rice straw

RI-Mesa

500

20

570

1.0 × 106

1.4 × 107

3.0 × 105

6.0 × 108

1.2 × 106

1.0 × 109

5.0 × 104

2.5 × 103

1.0 × 104

RI-MesaA

500

20

570

1.0 × 106

1.4 × 107

3.0 × 105

1.2 × 109

1.2 × 106

1.0 × 109

5.0 × 104

2.5 × 103

1.0 × 104

RI-MesaB

500

20

570

1.0 × 106

1.4 × 107

3.0 × 105

6.0 × 108

2.4 × 106

1.0 × 109

5.0 × 104

2.5 × 103

1.0 × 104

RI-MesaC

500

20

570

1.0 × 106

1.4 × 107

3.0 × 105

6.0 × 108

1.2 × 106

2.0 × 109

5.0 × 104

2.5 × 103

1.0 × 104

RI-MesaG

500

20

570

1.0 × 106

1.4 × 107

3.0 × 105

6.0 × 108

1.2 × 106

1.0 × 109

5.0 × 104

2.5 × 103

2.0 × 104

RI-Control

500

/

570

/

/

/

/

/

/

/

/

–

  1. aR reed straw, C corn straw, and RI rice straw; control group means the control without bacterial and enzyme supplementation; Mesa bacterial inoculant and enzyme silage additive (bacterial inoculants: 1.0 × 106 CFU L. plantarum, 1.4 × 107 CFU L. buchneri; 3.0 × 105 CFU P. pentosaceus; 8.0 × 108 CFU A. niger; 1.6 × 106 CFU B. subtilis; 1.0 × 109 CFU C. utilis; and enzymes: 5.0 × 104 U xylanase; 2.5 × 103 U β-Mannanases; 1.0 × 104 U glucanase), MesaA represents Mesa with a double or triple dosage of A. niger for reed, corn and rice straws, respectively, MesaB represents Mesa with a double dosage of B. subtilis, MesaC represents Mesa with a double dosage of C. utilis, MesaG represents Mesa with a double dosage of glucanase
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