Bacterial inoculants and enzymes based silage cocktails boost the ensiling quality of biomasses from reed, corn and rice straw

Chemical and Biological Technologies in Agriculture

Table 4 Effects of silage cocktails based on different combinations of bacterial inoculants and enzyme preparations on the nutritional value of straw silage

Biomass	Cocktails^d	CP, %	EE, %	GE, MJ/kg	NDF, %	ADF, %	ash, %
Reed straw	R-Control	1.34^c	4.90^b	17.75^b	87.73^a	62.51^a	6.11^a
	R-Mesa	2.27^b	5.77^a	17.95^a	82.19^b	57.30^b	5.38^b
	R-MesaA	2.26^b	5.76^a	18.04^a	82.89^b	57.63^b	5.41^b
	R-MesaB	2.39^a	5.82^a	18.02^a	79.94^c	57.59^b	5.39^b
	R-MesaC	2.42^a	5.82^a	18.03^a	83.63^b	58.66^b	5.26^b
	R-MesaG	2.29^b	5.77^a	18.04^a	83.18^b	57.95^b	5.43^b
	SEM	0.05	0.04	0.02	0.37	0.39	0.04
	P value	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01
Corn straw	C-Control	6.23^c	5.79^b	17.37^b	73.66^a	43.71^a	9.90^a
	C-Mesa	6.86^b	7.68^a	17.68^a	68.27^b	39.94^b	8.24^b
	C-MesaA	6.89^b	7.77^a	17.69^a	68.95^b	40.42^b	8.44^b
	C-MesaB	7.65^a	7.88^a	17.64^a	66.21^c	40.01^b	8.16^b
	C-MesaC	7.51^a	7.73^a	17.69^a	68.94^b	40.23^b	8.47^b
	C-MesaG	7.07^b	7.73^a	17.66^a	68.17^b	40.51^b	8.27^b
	SEM	0.07	0.11	0.02	0.40	0.66	0.09
	P value	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01
Rice straw	RI-Control	6.31^c	2.66^b	13.66^b	63.10^a	41.16^a	12.49^a
	RI-Mesa	7.67^b	3.74^a	13.82^a	55.77^a	36.81^b	11.42^b
	RI-MesaA	7.66^b	3.65^a	13.87^a	55.21^a	36.85^b	11.16^b
	RI-MesaB	7.83^a	3.65^a	13.87^a	53.55^c	34.59^c	11.30^b
	RI-MesaC	7.86^a	3.63^a	13.88^a	55.66^b	36.33^b	11.14^b
	RI-MesaG	7.68^b	3.61^a	13.84^a	55.70^b	36.59^b	11.21^b
	SEM	0.09	0.06	0.02	0.47	0.50	0.09
	P value	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01	< 0.01

CP crude protein, EE ether extract, GE gross energy, NDF neutral detergent fibre, ADF acid detergent fibre
^a–cMean the statistical differences in the same column within each straw type with a P value less than 0.05
^dR reed straw, C corn straw, and RI rice straw, control group means the control without bacterial and enzyme supplementation; Mesa the basal silage cocktail treatment ( containing 1.0 × 10⁶ CFU L. plantarum, 1.4 × 10⁷ CFU L. buchneri; 3.0 × 10⁵ CFU P. pentosaceus; 8.0 × 10⁸ CFU A. niger; 1.6 × 10⁶ CFU B. subtilis; 1.0 × 10⁹ CFU C. utilis; and enzymes: 5.0 × 10⁴ U xylanase; 2.5 × 10³ U β-Mannanases; 1.0 × 10⁴ U glucanase), MesaA represents Mesa with a double or triple dosage of A. niger for reed, corn and rice straws, respectively, MesaB represents Mesa with a double dosage of B. subtilis, MesaC represents Mesa with a double dosage of C. utilis, MesaG represents Mesa with a double dosage of glucanase