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Chemical and Biological Technologies in Agriculture
Fig. 6 | Chemical and Biological Technologies in Agriculture

Fig. 6

From: Innovative approach for semi‐continuous production of puerarin palmitate via transesterification with novel immobilized lipase

Fig. 6

a Solvent stability and b thermal stability of the immobilized lipases during p‐NPP hydrolysis. Reaction conditions: 10–40 mg/mL immobilized enzyme, 10 mM p‐NPP solution and lipase solution in 50 mM Tris‐HCl buffer (pH 8.0) in a 1:1 ratio (v/v), reaction temperature of 50 ℃, and reaction time of 20 min. Different letters above the bars indicate significant differences between immobilized lipases according to Tukey’s HSD test (P < 0.05)

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