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Table 1 Summary of reaction conditions for the enzymatic synthesis of flavonoid esters

From: Innovative approach for semi‐continuous production of puerarin palmitate via transesterification with novel immobilized lipase

Flavonoid

Acyl donor

Enzyme; enzyme concentration

Molar ratio (Flavonoid: Acyl donor)

Reaction temperature

Solvent

Reaction time; conversion rate

Refs.

Naringin

Oleic acid (C18)

Lipozyme TL IM; 10 g/L

1:20

40 °C

Acetonitrile

24 h; 92.17%

48 h; 93.10%

[3]

Naringin

Acetic anhydride, vinyl acetate (C2)

Lipozyme TL IM; 3 g/L

1:5

40 °C

Acetonitrile

8 h; 98.51%,

24 h; 97.54%

[4]

Naringin

Palmitic acid (C16)

Lipozyme TL IM; 50 g/L

1:10

50 °C

Acetone

16 h; ~ 90%

[26]

Naringin

Palmitic acid (C16)

Novozym 435; 10 g/L

1:5

60 °C

tert‐Amyl alcohol

45 h; 43%

[40]

Neohesperidin

Vinyl esters (C2, C12, C16)

Liozyme TL IM; 43 g/L

1:8 (C12, C16) 1:12 (C2)

52 °C

tert‐Amyl alcohol: DMSO = 4:1

24 h;

55% (C2),

83% (C12),

80% (C16)

[21]

Phloridzin, naringin, esculin

Oleic acid (C18)

CAL B immobilized onto a hydrophobic carrier; 2 g/L

1:18

65 °C

Acetonitrile

72 h;

76.93% (phloridzin),

75.43% (naringin),

78.11% (esculin)

[41]

Puerarin

Vinyl esters (C3-C8)

Whole‐cell (Aspergillus oryzae GIM 3.4826); 50 g/L

1:30

40 °C

Tetrahydrofuran

24 h;

94.20% (C3), 95.90% (C4), 60.60% (C5), 93.40% (C6), 46.70% (C8)

[52]

Puerarin

Vinyl esters (C3, C6, C14)

Novozym 435; 2 g/L

1:30

50 °C

1‐Ethyl‐3‐methylimidazoliumacetate

6 h;

63.91% (C3),

 ~ 40% (C6),

 ~ 35% (C14)

[53]

Puerarin

Vinyl palmitate (C16)

TL GASG; 27.9 g/L

1:15

40 °C

tert‐Amyl alcohol

3 h; 97.17%

This study