Fig. 4

Effect of type A or B agent treatment against CMV infections in C. annuum. A, C The plants inoculated by CMV-infected leaf tissue were observed under normal light. The treatments were initiated 5 days after virus inoculation, and additional treatments were performed at intervals of either 5 days (5 days) or 10 days (10 days). The ‘untreated’ control image was re-used to provide a separate illustration with each treatment. B, D The viral RNA of CMV in systemic leaves was detected by semi-quantitative RT-PCR using a CMV-specific primer set. 25c, 30c, and 35c indicate PCR cycles used for amplification. The gel images representing amplified fragments from the ‘untreated’ control were re-used to provide a separate illustration with each treatment