Chemical structure elucidation and functional activities comparison of two polysaccharides purified from Citrus reticulata Blanco peels

Li, Xiaojun; Chen, Jiang; Yin, Yuan; Xiao, Shijun; Zhang, Rui; Yang, Yang; Li, Le; Xu, Hairong; Zhang, Xue; Hu, Pei

doi:10.1186/s40538-024-00556-2

Chemical and Biological Technologies in Agriculture

Table 1 Methylation analysis and type of linkage of CRP-WA and CRP-02A

From: Chemical structure elucidation and functional activities comparison of two polysaccharides purified from Citrus reticulata Blanco peels

Glycosyl linkage	PMAA derivative	Area percentage (%)^*		m/z
Glycosyl linkage	PMAA derivative	CRP-WA	CRP-02A	m/z
T-Araf	1,4-Di-O-acetyl-2,3,5-tri-O-methyl-arabinitol	46.17	2.55	87, 101, 117, 129, 145, 161, 205
1,3-Araf	1,3,4-Tri-O-acetyl-2,5-di-O-methyl-arabinitol	0.56	n.d	87, 99, 117, 129, 159, 173, 189
1,5-Araf	1,4,5-Tri-O-acetyl-2,3-di-O-methyl-arabinitol	34.50	4.38	87, 101, 117, 129, 161, 173, 189
1,3,5-Araf	1,3,4,5-Tetra-O-acetyl-2-O-methyl-arabinitol	7.90	3.12	85, 99, 117, 127, 141, 159,172, 201, 261
Total Ara		89.13^a	10.05
1,4-Galp	1,4,5-Tri-O-acetyl-2,3,6-tri-O-methyl galactitol	n.d	61.49	87, 101, 117, 129, 161, 173, 233
1,3-Galp	1,3,5-Tri-O-acetyl-2,4,6-tri-O-methyl-galactitol	1.09	n.d	87, 101, 117, 129, 161, 173, 203, 233
1,6-Galp	1,5,6-Tri-O-acetyl-2,3,4-tri-O-methyl-galacitol	0.82	n.d	87, 101, 117, 129, 161, 173, 203, 217, 233
1,3,4-Galp	1,3,4,5-Tetra-O-acetyl-2,6-di-O-methyl galactitol	n.d	15.15	87, 93, 117, 129, 159, 171, 185, 203, 231, 245, 260, 305
1,4,6-Galp	1,4,5,6-Tetra-O-acetyl-2,3,-di-O-methyl galactitol	n.d	10.56	85, 101, 117, 129, 142, 159, 187, 201, 261, 272, 296, 314, 329
1,3,6-Galp	1,3,5,6-Tetra-O-acetyl-2,4-di-O-methyl galactitol	2.22	n.d	87, 101, 117, 129, 159, 173, 189, 233, 273
1,3,4,6,-Galp	1,3,4,5,6-Acetyl-2-O-methyl galactitol	n.d	2.75	77,87, 102,117, 139, 159, 175, 198, 219, 230, 266, 292, 312, 338, 369, 398
Total Gal		4.13^b	89.95
1,4-Manp	1,4,5-Tri-O-acetyl-2,3,6-Tri-O-methyl-mannitol	1.00	n.d	87, 99, 117, 129, 143, 161, 173, 203, 233, 281
Total Man		1.00^c
1,4-Glcp	1,4,5-Tri-O-acetyl-2,3,6-tri-O-methyl glucitol	5.74	n.d	87, 99, 117, 129, 161, 173, 189, 233, 253
Total Glc		5.74^d

^* The percentages reported are peak areas from the relative mass detector response (%). n.d = not detected; ^a shows the sum of area percentages of PMAA derivatives from L-arabinofuranosyl residue, ^b shows that from D-galactopyranosyl residue, ^c shows that from D-mannopyranosyl residue and ^d from D-glucopyranosyl residue.

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