Items | Treatments | Fermentation days | SEM | P-value | ||||||
---|---|---|---|---|---|---|---|---|---|---|
2 | 4 | 8 | 15 | 30 | T | D | T × D | |||
pH | CON | 4.27 | 3.76 | 3.71 | 3.67 | 3.70 | 0.026 |  < 0.001 |  < 0.001 |  < 0.001 |
LS | 3.78 | 3.75 | 3.70 | 3.57 | 3.56 | |||||
WA | 3.68 | 3.62 | 3.59 | 3.61 | 3.52 | |||||
Dry matter (g/kg fresh weight) | CON | 274 | 266 | 252 | 236 | 234 | 2.408 | 0.453 | 0.001 | 0.262 |
LS | 255 | 258 | 255 | 239 | 230 | |||||
WA | 257 | 242 | 246 | 250 | 240 | |||||
Lactic acid (g/kg dry matter) | CON | 56.5 | 70.8 | 94.7 | 130 | 143 | 4.265 | 0.427 |  < 0.001 | 0.217 |
LS | 81.5 | 83.4 | 90.3 | 98.5 | 119 | |||||
WA | 73.9 | 91.3 | 110 | 124 | 120 | |||||
Acetic acid (g/kg dry matter) | CON | 7.03 | 5.62 | 7.20 | 9.59 | 13.0 | 1.879 |  < 0.001 |  < 0.001 |  < 0.001 |
LS | 22.1 | 28.9 | 29.7 | 39.9 | 50.8 | |||||
WA | 13.1 | 15.0 | 16.5 | 18.1 | 16.9 | |||||
Lactic acid/acetic acid | CON | 8.07 | 12.7 | 13.2 | 13.5 | 11.2 | 0.241 |  < 0.001 |  < 0.001 |  < 0.001 |
LS | 3.69 | 2.89 | 3.02 | 2.47 | 2.32 | |||||
WA | 5.63 | 6.15 | 6.70 | 6.84 | 7.05 | |||||
Propionic acid (g/kg dry matter) | CON | 0.27 | 0.23 | 0.29 | 0.56 | 4.06 | 0.241 |  < 0.001 |  < 0.001 |  < 0.001 |
LS | 0.82 | 0.68 | 1.63 | 3.44 | 5.85 | |||||
WA | 1.08 | 1.16 | 1.27 | 1.16 | 1.60 | |||||
Butyric acid (g/kg dry matter) | CON | ND | ND | ND | ND | ND | – | – | – | – |
LS | ND | ND | ND | ND | ND | |||||
WA | ND | ND | ND | ND | ND | |||||
1,2-Propanediol (g/kg dry matter) | CON | ND | ND | ND | ND | ND | – | – | – | – |
LS | ND | ND | ND | ND | 2.08 | |||||
WA | ND | ND | ND | ND | ND | |||||
Ethanol (g/kg dry matter) | CON | 1.93 | 3.07 | 8.68 | 17.5 | 17.4 | 0.747 |  < 0.001 |  < 0.001 |  < 0.001 |
LS | 3.27 | 2.49 | 3.29 | 3.50 | 4.02 | |||||
WA | 2.70 | 3.45 | 7.47 | 8.48 | 8.49 | |||||
Water-soluble carbohydrates (g/kg dry matter) | CON | 179 | 168 | 157 | 131 | 101 | 3.093 |  < 0.001 |  < 0.001 |  < 0.001 |
LS | 176 | 168 | 158 | 147 | 125 | |||||
WA | 167 | 163 | 152 | 143 | 141 | |||||
Ammonia nitrogen (g/kg total nitrogen) | CON | 41.6 | 52.6 | 57.9 | 70.9 | 81.4 | 2.325 |  < 0.001 |  < 0.001 | 0.702 |
LS | 30.6 | 42.8 | 47.0 | 55.3 | 64.0 | |||||
WA | 23.2 | 31.8 | 37.1 | 45.7 | 55.5 |