Figure 3From: Stimulation of plant growth and biocontrol by Bacillus amyloliquefaciens subsp. plantarum FZB42 engineered for improved actionPCR analysis of FZBAN and FZBHarpin. (A) Validation of FZB42AN (FAN, DnprE DnprA, deficient in neutral and alkaline protease). Left: N, negative control (double distilled water); P, 844-bp fragment from the Km-containing plasmid; FAN 2 and FAN5, presence of the Km resistance cassette in two FZB42AN isolates. Right: N, negative control (double distilled water); P, 1,438-bp fragment from the Spec-containing plasmids; FAN 2 and FAN5, respectively, presence of the Spec resistance cassette in the two FZB42AN isolates. (B) Validation of FZBHarpin (FZBH, nprE::hpa1 nprA::hpa1). Lanes from left to right: presence of the spec resistance gene (1,438 bp) in FZB42Harpin, presence of the hpa1 gene (813 bp) in FZB42Harpin, presence of the his-tagged hpa1 gene (844 bp) in FZB42Harpin, and presence of the km resistance gene (880 bp) in FZB42Harpin.Back to article page