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Table 4 Strains and plasmids used for constructing FZB42Harpin

From: Stimulation of plant growth and biocontrol by Bacillus amyloliquefaciens subsp. plantarum FZB42 engineered for improved action

Strains/plasmids Description Reference or source
Plasmids
 pUC18 Cloning vector; lacZ Apr Lab collection
 pGEMT-easy T/A-clone site vector; lacZ; Apr Promega Corp. Fitchburg, Wisconsin
 pBT2-arcA Allelic replacement vector for Staphylococcus aureus containing a Km resistance cassette Leibig et al. [45]
 pIC333 A vector carrying mini-Tn10 transposase gene for Bacillus subtilis, offer of Spec cassette Laboratory stock
 pM43HF Expression vector carrying hpa1 gene under the control of promoter p43 and the nprB signal peptide Wu et al. [42]
 pGAprEHS pGEM-T carrying a 2.8-kb fragment containing aprE, a 1.4-kb fragment lox-Spec and a 0.8-kb fragment hpa1; Apr, Specr This study
 pGAprES pGEM-T carrying a 2.8-kb fragment containing aprE, a 1.4-kb fragment lox-Spec; Apr, Specr This study
 pUNprEKHS pUC18 plasmid carrying a lox-Km cassette, nprE-L, nprE-R fragment, hpa1-his fragment; ApR, KmR This study
 pUNprEK pUC18 plasmid carrying a lox-Km cassette, nprE-L fragment and nprE-R fragment; ApR, KmR This study
Strains
E. coli
  Topo10 F-mcrA Δ (mrr-hsdRMS-mcrBC φ 80lacZ Δ M15 Δ lacX74 nupG recA1 araD139 Δ (ara-leu)7697 galE15 galK16 rpsL(Str R ) endA1 λ Invitrogen
Bacillus amyloliquefaciens
  FZB42 Type strain for Bacillus amyloliquefaciens subsp. plantarum ABiTEP GmbH, Berlin, Germany
  FZB42/AHS FZB42aprEnprE:: hpa1:: lox-Spec This study
  FZBHarpin FZB42aprEnprE:: hpa1:: hpa1his:: lox-Spec::lox-Km This study
  FZBAN FZB42aprEnprE:: lox-Spec::lox-Km This study
Xanthomonas oryzae pv. oryzae POX99 This study