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Table 4 Strains and plasmids used for constructing FZB42Harpin
| Strains/plasmids | Description | Reference or source |
|---|---|---|
| Plasmids | ||
| pUC18 | Cloning vector; lacZ Apr | Lab collection |
| pGEMT-easy | T/A-clone site vector; lacZ; Apr | Promega Corp. Fitchburg, Wisconsin |
| pBT2-arcA | Allelic replacement vector for Staphylococcus aureus containing a Km resistance cassette | Leibig et al. [45] |
| pIC333 | A vector carrying mini-Tn10 transposase gene for Bacillus subtilis, offer of Spec cassette | Laboratory stock |
| pM43HF | Expression vector carrying hpa1 gene under the control of promoter p43 and the nprB signal peptide | Wu et al. [42] |
| pGAprEHS | pGEM-T carrying a 2.8-kb fragment containing aprE, a 1.4-kb fragment lox-Spec and a 0.8-kb fragment hpa1; Apr, Specr | This study |
| pGAprES | pGEM-T carrying a 2.8-kb fragment containing aprE, a 1.4-kb fragment lox-Spec; Apr, Specr | This study |
| pUNprEKHS | pUC18 plasmid carrying a lox-Km cassette, nprE-L, nprE-R fragment, hpa1-his fragment; ApR, KmR | This study |
| pUNprEK | pUC18 plasmid carrying a lox-Km cassette, nprE-L fragment and nprE-R fragment; ApR, KmR | This study |
| Strains | ||
| E. coli | ||
| Topo10 | F-mcrA Δ (mrr-hsdRMS-mcrBC φ 80lacZ Δ M15 Δ lacX74 nupG recA1 araD139 Δ (ara-leu)7697 galE15 galK16 rpsL(Str R ) endA1 λ − | Invitrogen |
| Bacillus amyloliquefaciens | ||
| FZB42 | Type strain for Bacillus amyloliquefaciens subsp. plantarum | ABiTEP GmbH, Berlin, Germany |
| FZB42/AHS | FZB42△aprE△nprE:: hpa1:: lox-Spec | This study |
| FZBHarpin | FZB42△aprE△nprE:: hpa1:: hpa1his:: lox-Spec::lox-Km | This study |
| FZBAN | FZB42△aprE△nprE:: lox-Spec::lox-Km | This study |
| Xanthomonas oryzae pv. oryzae POX99 | This study | |
