|
Plasmids
|
|
pUC18
|
Cloning vector; lacZ Apr
|
Lab collection
|
|
pGEMT-easy
|
T/A-clone site vector; lacZ; Apr
|
Promega Corp. Fitchburg, Wisconsin
|
|
pBT2-arcA
|
Allelic replacement vector for Staphylococcus aureus containing a Km resistance cassette
|
Leibig et al. [45]
|
|
pIC333
|
A vector carrying mini-Tn10 transposase gene for Bacillus subtilis, offer of Spec cassette
|
Laboratory stock
|
|
pM43HF
|
Expression vector carrying hpa1 gene under the control of promoter p43 and the nprB signal peptide
|
Wu et al. [42]
|
|
pGAprEHS
|
pGEM-T carrying a 2.8-kb fragment containing aprE, a 1.4-kb fragment lox-Spec and a 0.8-kb fragment hpa1; Apr, Specr
|
This study
|
|
pGAprES
|
pGEM-T carrying a 2.8-kb fragment containing aprE, a 1.4-kb fragment lox-Spec; Apr, Specr
|
This study
|
|
pUNprEKHS
|
pUC18 plasmid carrying a lox-Km cassette, nprE-L, nprE-R fragment, hpa1-his fragment; ApR, KmR
|
This study
|
|
pUNprEK
|
pUC18 plasmid carrying a lox-Km cassette, nprE-L fragment and nprE-R fragment; ApR, KmR
|
This study
|
|
Strains
|
|
E. coli
|
|
Topo10
|
F-mcrA
Δ
(mrr-hsdRMS-mcrBC
φ
80lacZ
Δ
M15
Δ
lacX74 nupG recA1 araD139
Δ
(ara-leu)7697 galE15 galK16 rpsL(Str
R
) endA1 λ
−
|
Invitrogen
|
|
Bacillus amyloliquefaciens
|
|
FZB42
|
Type strain for Bacillus amyloliquefaciens subsp. plantarum
|
ABiTEP GmbH, Berlin, Germany
|
|
FZB42/AHS
| FZB42△aprE△nprE:: hpa1:: lox-Spec
|
This study
|
|
FZBHarpin
| FZB42△aprE△nprE:: hpa1:: hpa1his:: lox-Spec::lox-Km
|
This study
|
|
FZBAN
| FZB42△aprE△nprE:: lox-Spec::lox-Km
|
This study
|
|
Xanthomonas oryzae pv. oryzae POX99
|
This study
|
