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Table 4 Strains and plasmids used for constructing FZB42Harpin

From: Stimulation of plant growth and biocontrol by Bacillus amyloliquefaciens subsp. plantarum FZB42 engineered for improved action

Strains/plasmids

Description

Reference or source

Plasmids

 pUC18

Cloning vector; lacZ Apr

Lab collection

 pGEMT-easy

T/A-clone site vector; lacZ; Apr

Promega Corp. Fitchburg, Wisconsin

 pBT2-arcA

Allelic replacement vector for Staphylococcus aureus containing a Km resistance cassette

Leibig et al. [45]

 pIC333

A vector carrying mini-Tn10 transposase gene for Bacillus subtilis, offer of Spec cassette

Laboratory stock

 pM43HF

Expression vector carrying hpa1 gene under the control of promoter p43 and the nprB signal peptide

Wu et al. [42]

 pGAprEHS

pGEM-T carrying a 2.8-kb fragment containing aprE, a 1.4-kb fragment lox-Spec and a 0.8-kb fragment hpa1; Apr, Specr

This study

 pGAprES

pGEM-T carrying a 2.8-kb fragment containing aprE, a 1.4-kb fragment lox-Spec; Apr, Specr

This study

 pUNprEKHS

pUC18 plasmid carrying a lox-Km cassette, nprE-L, nprE-R fragment, hpa1-his fragment; ApR, KmR

This study

 pUNprEK

pUC18 plasmid carrying a lox-Km cassette, nprE-L fragment and nprE-R fragment; ApR, KmR

This study

Strains

 E. coli

  Topo10

F-mcrA Δ (mrr-hsdRMS-mcrBC φ 80lacZ Δ M15 Δ lacX74 nupG recA1 araD139 Δ (ara-leu)7697 galE15 galK16 rpsL(Str R ) endA1 λ −

Invitrogen

 Bacillus amyloliquefaciens

  FZB42

Type strain for Bacillus amyloliquefaciens subsp. plantarum

ABiTEP GmbH, Berlin, Germany

  FZB42/AHS

FZB42â–³aprEâ–³nprE:: hpa1:: lox-Spec

This study

  FZBHarpin

FZB42â–³aprEâ–³nprE:: hpa1:: hpa1his:: lox-Spec::lox-Km

This study

  FZBAN

FZB42â–³aprEâ–³nprE:: lox-Spec::lox-Km

This study

Xanthomonas oryzae pv. oryzae POX99

This study