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Table 2 Total P (TP E ) and organic P (OP E ) in soil extracts (mg kg −1 ) and relative distribution of P

From: Optimized procedure for the determination of P species in soil by liquid-state 31P-NMR spectroscopy

P species

Phos-P

Orth-P

Monoesters

PL

DNA

Diesters a

Pyro-P

Poly-P

OP E

TP E

ppm intervals

18 to 24

5.6

5.3 to 3.3

1.6 to −0.3

0.8

−1.1 to −1.6

−4.6

−19 to −21

  

Farmland soil

Freeze-drying

0.5

116.7

14.7

0.6

1.0

1.6

0.2

0.1

16.8

133.8

Air-drying

ND

106.1

7.8

0.5

0.3

0.9

0.2

0.1

8.8

115.1

Fresh

1.4

98.7

8.4

0.9

0.3

1.1

0.2

0.1

11.0

109.9

Farmland soil added with compost

Freeze-drying

0.5

160.3

16.0

1.7

1.4

3.2

0.4

0.2

19.9

180.7

Air-drying

0.5

153.7

8.6

0.3

0.2

0.4

0.1

0.7

10.3

164.1

Fresh

0.3

153.8

12.8

1.6

0.6

2.2

0.8

0.7

16.0

170.5

Forest soil

Freeze-drying

4.1

172.0

74.8

3.0

2.5

5.5

0.9

0.4

84.9

257.9

Air-drying

4.5

159.1

70.7

2.0

2.1

4.1

1.0

0.2

79.5

239.7

Fresh

5.2

127.8

52.7

1.2

ND

1.2

ND

2.0

61.1

188.9

Forest litter

Freeze-drying

8.1

38.6

80.3

2.6

6.7

9.4

2.5

1.4

97.1

140.2

Air-drying

7.8

31.0

83.4

1.9

6.0

7.9

2.5

ND

99.1

132.7

Fresh

3.3

46.2

80.6

2.1

4.9

7.0

1.2

1.0

91.9

139.4

  1. Species in 31P-NMR spectra acquired for 15 h, as referred to TPE and expressed in milligrams per kilograms. Phos-P, phosphonates; Ortho-P, orthophosphate; Mono-esters, orthophosphate monoesters; PL, phospholipids; Diesters, orthophosphate diesters; Pyro-P, pyrophosphate; Poly-P, polyphosphate; OPE, organic phosphorus in extracts; TPE, total phosphorus in extracts; ND, not detected. aExtracted orthophosphate diesters were calculated by the sum of phospholipids and DNA.