Figure 8From: Humic substances stimulate maize nitrogen assimilation and amino acid metabolism at physiological and molecular level Analysis of the expression of NR, AS, CS, IDH and MDH in leaves. The measurements were performed by using semi-quantitative RT-PCR method in the linear range. The seedlings were grown in a control complete nutrient solution (1) and in a control N-deprived solution (3) for 14 days. One milligramme C L−1 of HS was added for 24 h after 13 days to both +N (2) and −N solutions (4). The specific transcript levels in the y-axis of histograms (top panel) are normalized against that of the reference gene and expressed in arbitrary units (AU).Back to article page