Fig. 1
From: The DINGGG thermoprotein is membrane bound in the Crenarchaeon Sulfolobus solfataricus
Intracellular localization of DING thermozyme by fluorescence microscopy. PARPSso was localized in the sulfolobal cell by using the primary anti-PARP-1 antibody and the secondary antibody coupled to Alexa Fluor 488. a top, from left to right side. Phase contrast image of three Sulfolobal cells; DAPI staining (blue) of nucleoids, and FM64 staining (red) of membrane of the same cells. Bottom. The same cells were stained with anti-PARP1 antibodies (green, on the left). DAPI/Anti-PARP merge (green/blue) and FM64/Anti-PARP merge (green/red) are also shown. b top. phase contrast image of an A375 melanoma cell (left) and DAPI staining of nucleus (right). Bottom. anti-PARP1 fluorescent staining (left) and merge of DAPI and anti-PARP1 staining (right). c top. phase contrast image of E. coli cells (left) and DAPI staining of nucleoids (right). Bottom. Anti-PARP1 staining (left) and merge of DAPI and anti-PARP1 staining (right)