Fig. 2
From: The DINGGG thermoprotein is membrane bound in the Crenarchaeon Sulfolobus solfataricus
PARPSso and DNA in soluble and membrane fractions. a SDS-PAGE (12 %). Coomassie (0.5 %)-stained gel; b anti-PARP 1 catalytic site immunoblotting; c Agarose (1 %) gel electrophoresis. L, cell lysate; FC, cytoplasm; FE, extrinsic membrane and FI, intrinsic membrane proteins; P, highly insoluble proteins (pellet). M molecular weight markers. Organic extraction of pellet P: d anti-PARP 1 catalytic site immunoblotting; e Agarose (1 %) gel electrophoresis. Butanolic (B), aqueous (W), Interface (I) phases; (Pden), insoluble pellet P suspension. 20 μg proteins and 200 ng DNA were loaded on gels