Fig. 3
From: The DINGGG thermoprotein is membrane bound in the Crenarchaeon Sulfolobus solfataricus
Nucleoid preparation from S. solfataricus lysate. a Sucrose (15–50 %) gradient profile. 1 ml fractions were analysed for UV absorption, at both 260 and 280 nm and pooled (R0 = fractions 1–2; R1 = fractions 3–6; R2 = fractions 7–10; R3 = fractions 11–15; R4 = fractions 16–19; R5 = fractions 20–22; R6 = fractions 23–27; R7 = fractions 28–32). b Anti-PARP-1 catalytic site immunoblotting of R0-R7 fractions (20 μg). c 0.7 % Agarose gel electrophoresis of pooled fractions. Samples were normalized per protein content (20 μg). In order to check that the stained bands were DNA, a parallel gel was run, loaded with gradient fractions before and after digestion with DNase I. In the lanes with the digested sample, only a weak small sized band (possible RNA) was present (data not shown)