Direct identification of Monilinia brown rot fungi on infected fruits by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry

Chemical and Biological Technologies in Agriculture

Table 2 Summary of all MALDI-TOF MS results generated for this study

	Species	Extracts	Correct ID (score)		Wrong ID	No ID
			>2.0	1.7–2.0
Agar	M. fructicola	30	30	0	0	0
	M. laxa	22	19	1	1	1
	M. fructigena/M. polystroma	36	30	3	0	3
Apple	M. fructicola	25	23	0	0	2
	M. laxa	25	24	0	0	1
	M. fructigena/M. polystroma	80	75	2	0	3
Prune	M. fructicola	7	5	1	0	1
	M. laxa	7	3	1	1	2
	M. fructigena/M. polystroma	33	24	2	3	4
Cherry	M. fructicola	31	31	0	0	0
	M. laxa	4	4	0	0	0
	M. fructigena/M. polystroma	21	21	0	0	0

Monilinia protein extracts were obtained from different sources (fungus grown on PDA plates or infected apples, prunes or cherries). Samples which did not yield a mass spectrum (less than 5 %) were excluded from this table. All species were identified with a microflex LT MALDI-TOF mass spectrometer (Bruker Daltonics GmbH) using the BioTyper software (Version 3.1, Bruker Daltonics GmbH) and a custom-made main spectra (MSP) reference database