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Table 2 Reconstitution media needed for reporter bacteria used in cellular biosensor tests

From: Light dazzles from the black box: whole-cell biosensors are ready to inform on fundamental soil biological processes

Whole-cell biosensor Reconstitution medium
P. putida KT2440 (pLYS24-davT-lux) M9 + benzoate + glucose with no NH4Cl
P. fluorescens DF5740E7, DF57-N3, DF57-11D1 Liquid Luria–Bertani medium + 0.9 % NaCl
P. fluorescens HK44 (pUTK21) Yeast–peptone–glucose extract + 1 M KCl
V. fischeri (BioTox® test) 2.0 % NaCl
C. metallidurans (BIOMET®) Tris salt medium (TSM) + Fe(NH4)3-citrate + gluconate
P. aureofaciens PGS12 Minimal Ayer culture medium + 25 mM HEPESa
R. leguminosarum 3841 (pOT1) Acid minimal salt (AMS) + thiamine
P. fluorescens A506 (pTS) M9 medium + thiamine + Casaminoacids + Noble agar
  1. a HEPES is the 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid