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Table 2 Reconstitution media needed for reporter bacteria used in cellular biosensor tests

From: Light dazzles from the black box: whole-cell biosensors are ready to inform on fundamental soil biological processes

Whole-cell biosensor

Reconstitution medium

P. putida KT2440 (pLYS24-davT-lux)

M9 + benzoate + glucose with no NH4Cl

P. fluorescens DF5740E7, DF57-N3, DF57-11D1

Liquid Luria–Bertani medium + 0.9 % NaCl

P. fluorescens HK44 (pUTK21)

Yeast–peptone–glucose extract + 1 M KCl

V. fischeri (BioTox® test)

2.0 % NaCl

C. metallidurans (BIOMET®)

Tris salt medium (TSM) + Fe(NH4)3-citrate + gluconate

P. aureofaciens PGS12

Minimal Ayer culture medium + 25 mM HEPESa

R. leguminosarum 3841 (pOT1)

Acid minimal salt (AMS) + thiamine

P. fluorescens A506 (pTS)

M9 medium + thiamine + Casaminoacids + Noble agar

  1. a HEPES is the 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid