Extraction method | L. perenne variety | Treatment (ml of extract per l of cell culture)a | Extraction method means | Extraction method × variety means | Variety means | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|
0 | 18.5 | 37 | 45.5 | 74 | 111 | 148 | |||||
Screw-pressing | Malone | 0.50 | 1.00 | 2.00 | 3.00 | 4.00 | 4.00 | 4.00 | 3.00 | 2.81 | |
Screw-pressing | Seagoe | 0.50 | 1.25 | 1.50 | 3.50 | 3.50 | 4.00 | 4.00 | 2.96 | 2.83 | |
Screw-pressing | Copeland | 0.50 | 1.25 | 2.50 | 3.50 | 3.50 | 4.00 | 4.00 | 3.13 | 2.79 | |
Screw-pressing (means) | 0.05 | 1.17 | 2.00 | 3.33 | 3.67 | 4.00 | 4.00 | 3.03 | |||
Homogenisation | Malone | 0.50 | 1.00 | 1.50 | 2.75 | 3.50 | 3.50 | 3.50 | 2.63 | ||
Homogenisation | Seagoe | 0.50 | 1.25 | 2.00 | 2.50 | 3.50 | 3.50 | 3.50 | 2.71 | ||
Homogenisation | Copeland | 0.50 | 1.25 | 1.50 | 2.50 | 3.00 | 3.00 | 3.50 | 2.46 | ||
Homogenisation (means) | 0.05 | 1.17 | 1.67 | 2.58 | 3.33 | 3.33 | 3.50 | 2.60 | |||
Treatment volume means | 0.05 | 1.17 | 1.83 | 2.96 | 3.50 | 3.67 | 3.75 |
ANOVA | d.f. | LSD (5 %) | Significance |
---|---|---|---|
Variety (V) | 2 | 0.176 | NS |
Extraction method (E) | 1 | 0.144 | *** |
Treatment volume (T) | 5 | 0.249 | *** |
V × E | 2 | 0.249 | NS (P = 0.059) |
V × T | 10 | 0.431 | NS |
E × T | 5 | 0.351 | * |
V × E × T | 10 | 0.609 | NS |
Residual | 108 |