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Table 1 Browning scores of bean cell suspension cultures, elicited for 24 h with extracts produced either by screw-pressing or homogenising L. perenne foliage in water

From: Bio-refining of perennial ryegrass (Lolium perenne): evaluation of aqueous extracts for plant defence elicitor activity using French bean cell suspension cultures

Extraction method

L. perenne variety

Treatment (ml of extract per l of cell culture)a

Extraction method means

Extraction method × variety means

Variety means

0

18.5

37

45.5

74

111

148

Screw-pressing

Malone

0.50

1.00

2.00

3.00

4.00

4.00

4.00

 

3.00

2.81

Screw-pressing

Seagoe

0.50

1.25

1.50

3.50

3.50

4.00

4.00

 

2.96

2.83

Screw-pressing

Copeland

0.50

1.25

2.50

3.50

3.50

4.00

4.00

 

3.13

2.79

Screw-pressing (means)

 

0.05

1.17

2.00

3.33

3.67

4.00

4.00

3.03

  

Homogenisation

Malone

0.50

1.00

1.50

2.75

3.50

3.50

3.50

 

2.63

 

Homogenisation

Seagoe

0.50

1.25

2.00

2.50

3.50

3.50

3.50

 

2.71

 

Homogenisation

Copeland

0.50

1.25

1.50

2.50

3.00

3.00

3.50

 

2.46

 

Homogenisation (means)

 

0.05

1.17

1.67

2.58

3.33

3.33

3.50

2.60

  

Treatment volume means

 

0.05

1.17

1.83

2.96

3.50

3.67

3.75

   

ANOVA

d.f.

LSD (5 %)

Significance

Variety (V)

2

0.176

NS

Extraction method (E)

1

0.144

***

Treatment volume (T)

5

0.249

***

V × E

2

0.249

NS (P = 0.059)

V × T

10

0.431

NS

E × T

5

0.351

*

V × E × T

10

0.609

NS

Residual

108

  
  1. NS not significant
  2. * P < 0.05, *** P < 0.001
  3. aAll six extracts were equalised to the protein content of the lowest sample (Copeland homogenised), as determined with the Folin reagent, by dilution with deionised water prior to their use to elicit the cells