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Table 1 Browning scores of bean cell suspension cultures, elicited for 24 h with extracts produced either by screw-pressing or homogenising L. perenne foliage in water

From: Bio-refining of perennial ryegrass (Lolium perenne): evaluation of aqueous extracts for plant defence elicitor activity using French bean cell suspension cultures

Extraction method L. perenne variety Treatment (ml of extract per l of cell culture)a Extraction method means Extraction method × variety means Variety means
0 18.5 37 45.5 74 111 148
Screw-pressing Malone 0.50 1.00 2.00 3.00 4.00 4.00 4.00   3.00 2.81
Screw-pressing Seagoe 0.50 1.25 1.50 3.50 3.50 4.00 4.00   2.96 2.83
Screw-pressing Copeland 0.50 1.25 2.50 3.50 3.50 4.00 4.00   3.13 2.79
Screw-pressing (means)   0.05 1.17 2.00 3.33 3.67 4.00 4.00 3.03   
Homogenisation Malone 0.50 1.00 1.50 2.75 3.50 3.50 3.50   2.63  
Homogenisation Seagoe 0.50 1.25 2.00 2.50 3.50 3.50 3.50   2.71  
Homogenisation Copeland 0.50 1.25 1.50 2.50 3.00 3.00 3.50   2.46  
Homogenisation (means)   0.05 1.17 1.67 2.58 3.33 3.33 3.50 2.60   
Treatment volume means   0.05 1.17 1.83 2.96 3.50 3.67 3.75    
ANOVA d.f. LSD (5 %) Significance
Variety (V) 2 0.176 NS
Extraction method (E) 1 0.144 ***
Treatment volume (T) 5 0.249 ***
V × E 2 0.249 NS (P = 0.059)
V × T 10 0.431 NS
E × T 5 0.351 *
V × E × T 10 0.609 NS
Residual 108   
  1. NS not significant
  2. * P < 0.05, *** P < 0.001
  3. aAll six extracts were equalised to the protein content of the lowest sample (Copeland homogenised), as determined with the Folin reagent, by dilution with deionised water prior to their use to elicit the cells