Table 2 Overview of MALDI-TOF MS studies of agriculturally relevant fungi
Samples/organisms | Highlights | Reference |
|---|---|---|
Alternaria | Detection of the Alternaria mycotoxins alternariol, alternariol monomethyl ether, and tentoxin by MALDI-TOF MS | Sivagnanam et al. [67] |
Alternaria | Separation of A. dauci, A. porri, A. solani, and A. tomatophila into three clusters by molecular analyses and MALDI-TOF MS | Brun et al. [102] |
Alternaria | Identification of 60 isolates of 12 Alternaria species by intact cell MALDI-TOF MS with small mycelium samples | Chowdappa et al. [103] |
Aspergillus | Optimization of protein extraction for 24 Aspergillus species from as few as 10,000 spores and identification of 11 proteins | Sulc et al. [104] |
Aspergillus | Identification of 12 Aspergillus strains by preparing crude extracts by bead beating | Hettick et al. [105] |
Aspergillus | Characterisation of A. ibericus strains by MALDI-TOF MS and comparison with related species | Kallow et al. [106] |
Aspergillus | Analysis of several aflatoxigenic and non-aflatoxigenic strain belonging to four Aspergillus species | Li et al. [107] |
Aureobasidium | Analysis of extracellular liamocins (mannitol oils) produced by A. pullulans | Price et al. [108] |
Aureobasidium | Determination of oil structures of different A. pullulans strains | Manitchotpisit et al. [109] |
Beer spoilage microorganisms | Detection and distinction of beer spoilage yeasts and bacteria from brewing yeasts | Turvey et al. [19] |
Bremia, Oidium | Identification of ribosomal proteins and histones as markers for the biotyping of plant pathogens | Beinhauer et al. [97] |
Chalara | In vitro and in vivo identification of C. fraxinea by secondary metabolites collected in methanol extracts | Pham et al. [110] |
Clonostachys | Cluster analysis of MALDI-TOF MS data of 45 Clonostachys strains from different substrates | Abreu et al. [111] |
Downy and powdery mildews | Identification of the obligate biotrophic mildew fungi Bremia lactucae and Oidium neolycopersici, also from infected leaves | Chalupova et al. [96] |
Fusarium | Identification and characterisation of F. verticillioides and fumonisins by MALDI-TOF MS and MALDI-TOF MS/MS | Chang et al. [112] |
Fusarium | Differentiation of Fusarium subspecies based on spores collected and prepared from isolates | Marchetti-Deschmann et al. [113] |
Fusarium | Optimisation of MALDI biotyping of three Fusarium species (16 isolates) and identification of proteins following on-target tryptic digestion | Dong et al. [114] |
Fusarium | Optimized sample preparation for strongly colored Fusarium conidia | Dong et al. [115] |
Fusarium | Mixed volume spore preparation for five Fusarium species | Kemptner et al. [116] |
Fusarium | Differentiation of Fusarium species with ferulic acid as the matrix and the dried-droplet technique | Kemptner et al. [117] |
Gibberella | Characterisation of G. zeae conidia by on-target trypsin digestion | Dong et al. [118] |
Metarhizium | Reference spectra for distinguishing 51 isolates of the M. anisopliae species complex | Lopes et al. [119] |
Monilinia | Identification of Monilinia brown rot fungi directly from infect fruits | Freimoser et al. [18] |
Monilinia | Identification distinction of four Monilinia species cultivated in vitro | Horka et al. [120] |
Penicillium | Discrimination of 12 Penicillium species based on crude extracts obtained by bead beating | Hettick et al. [121] |
Penicillium | Six Penicillium species directly detected on citrus and apple fruits | Chen et al. [122] |
Puccinia | Identification of different species and pathotypes of P. triticina and P. graminis by intact spore MALDI-TOF MS | Beinhauer et al. [123] |
Rhizopus, Trichoderma, Phanerochaete | Comparison of sample preparation, matrices, and double-stick tape for collection of fungal material | Valentine et al. [124] |
Saccharomyces | Fingerprinting of 33 Saccharomyces strains commonly used for wine fermentation | Usbeck et al. [88] |
Saccharomyces | Comparison of SAPD-PCR (specifically amplified polymorphic DNA) and MALDI-TOF MS for identifying related Saccharomyces species | Blattel et al. [72] |
Saccharomyces | Identification of yeasts involved in chichi fermentation | Vallejo et al. [98] |
Saccharomyces | MALDI-TOF MS characterization of protein biomarkers desorbed from S. cerevisiae by formic acid | Amiri-Eliasi et al. [125] |
Sepedonium | Characterisation of mycoparasitic Sepedonium species and analysis of low-molecular weight peptides | Neuhof et al. [126] |
Spoilage yeasts | Optimization of MALDI-TOF MS assay for Saccharomyces, Wickerhamomyces and Debaryomyces isolated from beverages | Usbeck et al. [87] |
Trichoderma | Analysis of 129 Trichoderma strains by MALDI-TOF MS as well as ITS and tef1 sequencing | De Respinis et al. [127] |
Trichoderma | Characterisation and clustering of Trichoderma strains, their peptaibiotics, and hydrophobins | Degenkolb et al. [128] |
Trichoderma | Detection of peptaibols in 28 Trichoderma species | Neuhof et al. [129] |
Trichoderma | Direct identification of hydrophobins in Trichoderma isolates by MALDI-TOF MS | Neuhof et al. [130] |
Trichoderma, Rhizoctonia | Visualization of metabolites produced during the antagonistic interaction of T. atroviride and R. solani | Holzlechner et al. [131] |
Verticillium | Identification of six pathogenic Verticillium isolates with a protocol involving sonication | Tao et al. [132] |
Wood decay fungi | Differentiation of closely related indoor wood decay fungi by MALDI-TOF MS (Serpula lacrymans, S. himantioides, Coniophora puteana, C. marmorata, and Antrodia vaillantii, A. sinuosa) | Schmidt and Kallow [133] |
Yeasts and filamentous fungi | MALDI lipid phenotyping as an alternative method for characterizing and identifying fungi | Stübiger et al. [134] |
Yeasts | Identification of food-borne yeasts (≥33 species, 96 isolates) by MALDI-TOF MS and conventional methods | Pavlovic et al. [99] |