Effect of arbuscular mycorrhizal fungi and water stress on ultrastructural change of glandular hairs and essential oil compositions in Ocimum gratissimum

Chemical and Biological Technologies in Agriculture

Table 2 Influence of mycorhization and water stress on essential oils yield and composition in O. gratissimum L

Treatments	NM.NS	NM.S	M.NS	MS
Oils content (%)	0.22 ± 0.03a	0.24 ± 0.02a	0.3 ± 0.1b	0.33 ± 0.08c

Components	Peak area %
	Hydrocarbons monoterpenes
Azulene	0.44 ± 0.02a	0.89 ± 0.03b	0.29 ± 0.06a	0.23 ± 0.07a
	Oxygenated monoterpenes
Eucalyptol	2.02 ± 0.08a	0.39 ± 0.06b	0.74 ± 0.11c	0.88 ± 0.05c
α-Campholene	0.20 ± 0.05a	0.26 ± 0.03a	0.23 ± 0.02a	0.13 ± 0.02b
Methyl-chavicol	85.26 ± 0.32a	84.38 ± 0.72a	86.40 ± 0.62a	92.62 ± 0.42b
Trans anethole	4.25 ± 0.22a	2.28 ± 0.3b	8.31 ± 0.41c	2.45 ± 0.38b
Cis anéthole	0.12 ± 0.04a	0.08 ± 0.03a	1.64 ± 0.14b	0.15 ± 0.05a
Total	91.85a	87.38b	97.32c	96.23c
	Hydrocarbons sesquiterpenes
Caryophyllene	0.21 ± 0.11a	0.36 ± 0.09a	0.11 ± 0.02b	0.15 ± 0.04b
Calarene	0.50 ± 0.15a	0.68 ± 0.12a	0.18 ± 0.14b	0.27 ± 0.11b
α-Longipinene	0.82 ± 0.18a	1.37 ± 0.09b	0.35 ± 0.18c	0.71 ± 0.16a
α-Caryophyllene	0.52 ± 0.19a	0.78 ± 0.02b	0.31 ± 0.16a	0.34 ± 0.18a
Aristolene	0.73 ± 0.14a	0.05 ± 0.2a	0.33 ± 0.08b	0.64 ± 0.1a
Germacrene-d	_	1.76 ± 0.18	_	_
β Cedrene	0.21 ± 0.11a	0.38 ± 0.09a	0.09 ± 0.02b	0.16 ± 0.08a
Aromadendrene	0.21 ± 0.13a	0.65 ± 0.15b	0.11 ± 0.09a	0.18 ± 0.09a
Cadinene	0.78 ± 0.19a	2.38 ± 0.09b	0.48 ± 0.32a	0.84 ± 0.11a
Total	3.98a	8.41b	1.96c	3.29a
	Oxygenated sesquiterpenes
Cubenol	–	0.20 ± 0.07	–	–

One-way analysis of variance was carried out for each parameter studied. Tukey’s post hoc multiple mean comparison test was used to test for significant differences between treatments (at p ≤ 0.05%). Univariate analysis was used to test significant differences in treatments, accessions and their interaction for an individual parameter. All statistical analyses were performed with IBM.SPSS statistics, Version 19.The results of each experiment (biochemical essays) were repeated three times and (20 times for mycorrhizal intensity essays)
The values are the average of 3 determinations, and the values followed by different letters are significantly different (p ≤ 0.05)