The biostimulant manufactured using diazotrophic endophytic bacteria and humates is effective to increase sugarcane yield

Chemical and Biological Technologies in Agriculture

Table 4 Effect of time of foliar application of biostimulant on sugarcane yield of plant growth and the first and second ratoons

	Stalk yield (Mg ha⁻¹)
	Control	60 dap	90 dap	60 + 90 dap
	Plant growth
	72.0c	98.5a	86.7b	93.0a
Increase in relation to the control (%)	–	37%	20%	29%
C.V. (%)	6.6	–	–	–
	1st ratoon
	63.9	66.9	66.2	65.0
C.V. (%)	6.15	–	–	–
	2nd ratoon
	67.1c	83.4a	78.1b	82.3a
Increase in relation to the control (%)	–	24%	16%	23%
C.V. (%)	6.2	–	–	–

The treatments consist of the application of water (control) and biostimulant at a rate of 450 L ha⁻¹, applied one time at 60 and 90 days after planting and two times (60 plus 90 days after planting, dap)
Average values followed by different letters are distinct by Fisher’s LSD test (p < 0.05). The increase in relation to the control was calculated by 100 * (x – y)/y, where x is the average of treatment and y is the average of control. C.V. (%) is the coefficient of variation of the experiment