Antibody production and application for immunoassay development of environmental hormones: a review

Chemical and Biological Technologies in Agriculture

Table 2 Common methods of coupling hapten derivative with carrier protein

Compound	Coupling methods	References
Estradiol	Carbon imide method	[110]
Estradiol	Active ester method	[38]
Diethylstilbestrol 19-nortestosterone	Mixed anhydride method	[87]
Medroxyprogesterone acetate	O-Carboxymethyl hydroxylamine method	[99]