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Table 3 Fe recovered (%) from iron–humate complexes formed with HA1, after a first (I) and a second (II)a extraction after different shaking times (h), with a solution of a DFOM siderophore and a mixture of four organic acids: citric, oxalic, tartaric and ketoglutaric acids (Mix) at different concentrations

From: Potential alteration of iron–humate complexes by plant root exudates and microbial siderophores

Extractant h Concentration
0.01 M 0.001 M 0.0001 M
I II I II I II
DFOM 1 24.3 ab 13.9 a 18.5 bcd 11.4 a 6.1 cde 2.1 a
2 32.8 b 20.4 ab 21.6 defg 12.3 ab 6.4 cde 1.9 a
4 53.6 c 22.6 ab 24.1 defgh 15.3 abc 6.9 de 1.9 a
16 57.6 c 26.4 ab 24.9 efgh 19.6 cdef 6.7 de 1.9 a
Mix 1 68.4 cde 53.9 c 14.4 abc 12.6 ab 6.3 cde 5.0 bc
2 77.2 def 65.3 cd 25.5 fgh 15.1 abc 7.0 de 5.5 bcd
4 86.1 ef 95.2 f 28.5 hi 19.1 cde 7.3 e 6.5 cde
16 88.2 f 78.9 def 33.1 i 26.6 gh 6.8 de 4.4 b
  1. All values are average of three replications. Values followed by the same letter within columns are not significantly different by Tukey’s test at the level of P ≤ 0.05
  2. aFe recovered after the second extraction was calculated on the basis of iron left in the solid iron–humates after the first extraction