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Table 4 Independent variables and their levels for Box–Behnken design

From: Statistical optimization of chitinase production by Streptomyces rubiginosus SP24 and efficacy of purified chitinase to control root-knot nematode infection in Vigna radiata under controlled conditions

Factor Name Low actual High actual Low coded High coded
A Colloidal chitin 0.1 1 − 1 1
B Xylose 0.2 2 − 1 1
C Peptone 0.2 1 − 1 1
D Dihydrogen potassium phosphate 0.1 0.4 − 1 1