Statistical optimization of chitinase production by Streptomyces rubiginosus SP24 and efficacy of purified chitinase to control root-knot nematode infection in Vigna radiata under controlled conditions

Chemical and Biological Technologies in Agriculture

Table 6 Results of ANOVA for response surface quadratic model

Source	Sum of squares	df	Mean square	F-value	p value Prob > F
Model	15.64013333	10	1.56401333	9.352075121	< 0.0001
A colloidal chitin	8.200533333	1	8.20053333	49.03539	< 0.0001
B xylose	2.6508	1	2.6508	15.85055588	0.0009
C peptone	2.3232	1	2.6508	13.89165965	0.0015
D Dihydrogen potassium phosphate	0.3468	1	0.3468	2.073703326	0.1670
AB	1.0404	1	1.0404	6.221109979	0.0226
AC	0.1225	1	0.1225	0.732493245	0.4033
AD	0.3721	1	0.3721	2.224985605	0.1531
BC	0.1369	1	0.1369	0.818598574	0.3775
BD	0.3025	1	0.3025	1.808809851	0.1954
CD	0.1444	1	0.1444	0.863445099	0.3651
Residual	3.010266667	18	0.16723704
Lack of fit	1.364346667	14	0.09745333	0.236836136	0.9810
Pure error	1.64592	4	0.41148
Cor total	18.6504	28

R-Squared 0.838595, Adj R-Squared 0.748926, Pred R-Squared 0.662642, Adeq Precision 11.52083