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Table 2 Screening of EPS-producing bacterial strains on solid media composed by Salts Agar Base (SAB) with nutrient mixtures of vinasse (1, 5 or 10%) and sucrose (0, 0.5, 1, 1.5, 2 or 5%) as carbon sources with and without yeast extract (0.4%)

From: Bioprospecting of exopolysaccharide-producing bacteria from different natural ecosystems for biopolymer synthesis from vinasse

Composition of nutrient mixtures added to SAB Number of positive strainsa
Vinasse (mL/L) Sucrose (g/L) Yeast extract (g/L)
10 0 0 3
10 0 4 3
10 5 0 7
10 10 0 7
10 15 0 8
10 20 0 10
10 50 0 13
50 0 0 3
50 0 4 3
50 5 0 3
50 10 0 3
50 15 0 3
50 20 0 4
50 50 0 6
100 0 0 0
100 0 4 0
100 5 0 0
100 10 0 0
100 15 0 0
100 20 0 0
100 50 0 0
  1. V: Vinasse; 10 mL/L = 1%, 50 mL/L = 5%, 100 mL/L = 10%
  2. S: Sucrose; 0 g/L = 0%, 5 g/L = 0.5%, 10 g/L = 1%, 15 g/L = 1.5%, 20 g/L = 2%, 50 g/L = 5%
  3. Y: Yeast extract; 0 g/L = 0%, 4 g/L = 0.4%
  4. aNumber of bacterial strains that grew as ropy colonies on solid media within 48 h of incubation at 30 °C