Bioprospecting of exopolysaccharide-producing bacteria from different natural ecosystems for biopolymer synthesis from vinasse

Chemical and Biological Technologies in Agriculture

Table 2 Screening of EPS-producing bacterial strains on solid media composed by Salts Agar Base (SAB) with nutrient mixtures of vinasse (1, 5 or 10%) and sucrose (0, 0.5, 1, 1.5, 2 or 5%) as carbon sources with and without yeast extract (0.4%)

Composition of nutrient mixtures added to SAB			Number of positive strains^a
Vinasse (mL/L)	Sucrose (g/L)	Yeast extract (g/L)	Number of positive strains^a
10	0	0	3
10	0	4	3
10	5	0	7
10	10	0	7
10	15	0	8
10	20	0	10
10	50	0	13
50	0	0	3
50	0	4	3
50	5	0	3
50	10	0	3
50	15	0	3
50	20	0	4
50	50	0	6
100	0	0	0
100	0	4	0
100	5	0	0
100	10	0	0
100	15	0	0
100	20	0	0
100	50	0	0

V: Vinasse; 10 mL/L = 1%, 50 mL/L = 5%, 100 mL/L = 10%
S: Sucrose; 0 g/L = 0%, 5 g/L = 0.5%, 10 g/L = 1%, 15 g/L = 1.5%, 20 g/L = 2%, 50 g/L = 5%
Y: Yeast extract; 0 g/L = 0%, 4 g/L = 0.4%
^aNumber of bacterial strains that grew as ropy colonies on solid media within 48 h of incubation at 30 °C