Evaluation of Alpinia galanga (Zingiberaceae) extracts and isolated trans-cinnamic acid on some mosquitoes larvae

Chemical and Biological Technologies in Agriculture

Table 3 Enzyme activity of surviving mosquito larvae after 24 h exposure to A. galanga extracts or trans-cinnamic acid

Compound^c	Acetylcholinesterase^a,b			Carboxylesterase^a,b			Glutathione-S-transferase^a,b
Compound^c	Ae. aegypti	A. dirus B	Cx. quinquefasciatus	Ae. aegypti	A. dirus B	Cx. quinquefasciatus	Ae. aegypti	A. dirus B	Cx. quinquefasciatus
C	13.80 ± 0.01a	12.72 ± 0.01b	13.94 ± 0.03c	110.96 ± 0.12b	125.10 ± 0.07e	107.70 ± 0.06e	2.99 ± 0.27a	4.35 ± 0.39a	5.60 ± 0.06e
H	14.62 ± 0.06c	13.46 ± 0.04c	15.71 ± 0.03e	124.46 ± 0.07e	104.47 ± 0.12d	85.55 ± 0.08d	4.44 ± 0.40b	6.21 ± 0.07b	5.04 ± 0.03e
D	15.91 ± 0.01d	14.08 ± 0.02e	13.67 ± 0.02b	112.73 ± 0.08c	101.56 ± 0.05c	76.02 ± 0.08b	5.42 ± 0.04e	6.96 ± 0.68b	5.13 ± 0.01b
E	17.00 ± 0.01e	13.53 ± 0.01d	15.23 ± 0.01d	117.44 ± 0.10d	95.74 ± 0.08a	72.88 ± 0.03a	5.32 ± 0.04d	6.21 ± 0.10b	5.40 ± 0.04d
M	22.85 ± 0.08f	14.76 ± 0.01f	16.52 ± 0.01f	108.05 ± 0.07a	100.85 ± 0.03b	73.82 ± 0.01a	6.13 ± 0.08f	5.66 ± 0.11c	4.91 ± 0.05a
Cin	13.73 ± 0.02b	12.52 ± 0.02a	12.99 ± 0.04a	127.91 ± 0.09f	104.35 ± 0.08d	79.64 ± 0.06c	4.93 ± 0.04c	12.65 ± 0.17d	5.53 ± 0.04c

^aFor all experiments, results followed by the same letters within the same column are not significantly different by Tukey’s test (P < 0.05)
^bUnits of enzymes: Acetylcholinesterase = μM acetylcholinesterase/min/mg of protein; carboxylesterase, nM p-nitrophenol/min/mg protein; glutathione-S-transferase, mM CDNB conjugated product/mg protein/min
^cCompounds: C, Comtrol, H, hexane crude extract; D, dichloromethane crude extract; E, ethyl acetate crude extract; M, methanol crude extract; Cin, trans-cinnamic acid