Skip to main content

Table 1 Maximum PHB content (%) accumulated by C. necator DSM 13513 grown in LB medium supplemented with mixture of VFAsynthetic, VFAextracted in different cultivation conditions

From: Polyhydroxyalkanoates (PHAs) from dairy wastewater effluent: bacterial accumulation, structural characterization and physical properties

Operating mode Carbon source and aeration conditions PHB contenta (%) Time (h)
bBatch culture VFAsynthetic Under shaken in aerobic condition 0.31 ± 0.001E 24
VFAextracted Under shaken in aerobic condition 0.48 ± 0.006D 48
Without VFA Under shaken in aerobic condition 0.025 ± 0.002F 24
cFed-batch fermentation VFAsynthetic No aeration 0.81 ± 0.07B 48
Aeration 0.60 ± 0.01C 48
12 h of aeration 1.34 ± 0.02A 48
VFAextracted 12 h of aeration 0.52 ± 0.03CD 48
  1. a The values represent the means ± SD of three replicates. Different letters indicate significant differences (P < 0.05)
  2. bLB medium (600 mL) with 2% (v/v) of VFAsynthetic, VFAextracted or without VFA (control). Incubation at 30 °C for 24 or 48 h under shaken in aerobic condition at 200 rpm
  3. cLB medium (4 L) with 2% (v/v) of VFAsynthetic or VFAextracted. pH at 6.5. Incubation at 30 °C for 48 h with agitation speed set at 200 rpm. Feeding at 2% (v/v) added at 3, 6, 9 and 12 h. Aeration: air sparging at 1 vvm; 12 h of aeration: air sparging at 1 vvm for 12 h followed by no aeration; no aeration