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Table 1 Comparison of homologous and heterologous ELISA

From: Heterologous immunoassay strategy for enhancing detection sensitivity of banned dye rhodamine B in fraudulent food

Ab Coating Ag Method Amax IC50 (ng/mL) LOD (ng/mL) Detectable range (ng/mL)
Ab1 RB–OVA icELISA 1.01 a/ / /
Ab1 RBITC–OVA icELISA 1.09 1.0 0.051 0.15 ~ 6.72
Ab2 RB–OVA icELISA 1.03 0.09 0.002 0.008 ~ 1.15
Ab2 RBITC–OVA icELISA 0.97 0.82 0.11 0.23 ~ 2.91
Ref Ab [27] RBITC–OVA bdcELISA / 0.4  < 10 0.10 ~ 1.80
Ref Ab [4] RB–OVA icELISA / 0.94 0.1 0.10 ~ 20.00
  1. a/, unavailable; bdcELISA, direct competitive ELISA
  2. italic values indicate the IC50 and LOD values achieved by the best Ag-Ab pairing