Table 1 Comparison of homologous and heterologous ELISA
Ab | Coating Ag | Method | Amax | IC50 (ng/mL) | LOD (ng/mL) | Detectable range (ng/mL) |
|---|---|---|---|---|---|---|
Ab1 | RB–OVA | icELISA | 1.01 | a/ | / | / |
Ab1 | RBITC–OVA | icELISA | 1.09 | 1.0 | 0.051 | 0.15 ~ 6.72 |
Ab2 | RB–OVA | icELISA | 1.03 | 0.09 | 0.002 | 0.008 ~ 1.15 |
Ab2 | RBITC–OVA | icELISA | 0.97 | 0.82 | 0.11 | 0.23 ~ 2.91 |
Ref Ab [27] | RBITC–OVA | bdcELISA | / | 0.4 | < 10 | 0.10 ~ 1.80 |
Ref Ab [4] | RB–OVA | icELISA | / | 0.94 | 0.1 | 0.10 ~ 20.00 |