Skip to main content
Chemical and Biological Technologies in Agriculture

Table 1 Primer set used to amplify bacterial and fungal communities

From: Cooperation among phosphate-solubilizing bacteria, humic acids and arbuscular mycorrhizal fungi induces soil microbiome shifts and enhances plant nutrient uptake

Primer pair

Primer

Sequence (5ʹ-3ʹ)*

Taxonomic group

Annealing temp (°C)

F984GC/R1378

F984

GC clamp-AACGCGAAGAACCTTAC

Bacteria

53

R1378

CGGTGTGTACAAGGCCCGGGAACG

  

ITS1F/ITS4

ITS1F

CTTGGTCATTTAGAGGAAGTAA

Fungi

55

ITS4

TCCTCCGCTTATTGATATGC

  
  1. *The Gc clamp sequence was CGCCCGGGGCGCGCCCCGGGCGGGGCGGGGGCACGGGGGG
Back to article page