Fig. 5

Comparison of the sensitivity of CBB staining methods using BA as a standard. From naked-eye observation, a band can be observed up to 74.1 ng for all staining methods. However, through ImageJ analysis, a peak can be observed in the plot profile up to 24.7 ng for both the improved and standard colloidal CBB-G staining methods. In contrast, for CBB-R staining, a peak can be observed only up to 74.1 ng. For the three staining methods, the plot profiles of the 2000.0 ng, 666.7 ng, and 222.2 ng bands were vertically scaled to 1.0, whereas the plot profiles of the 74.1 ng, 24.7 ng, and 8.2 ng bands were vertically scaled to 2.0