Table 1 Comparison of the total time needed to perform the three CBB staining methods
Step | Staining method | ||
|---|---|---|---|
CBB-R | Standard CBB-G | Improved CBBG | |
Fixation | – | – | 30 min |
3× Wash | – | 10 min × 3 | 10 min × 3 |
Staining | 30Â min to overnight | 2Â h to overnight | 2Â h to overnight |
Destaining | 1–3 h | 10–60 min | 3–5 min |
Washing | – | Rinse | 10 min |
Fastest total time | 1Â h 30Â min | 2Â h 40Â min | 3Â h 13Â min |