Effects of Lactiplantibacillus plantarum inoculation on the quality and bacterial community of whole-crop corn silage at different harvest stages

Dong, Jianan; Li, Songze; Chen, Xue; Sun, Zhe; Sun, Yue; Zhen, Yuguo; Qin, Guixin; Wang, Tao; Demelash, Natnael; Zhang, Xuefeng

doi:10.1186/s40538-022-00326-y

Chemical and Biological Technologies in Agriculture

Table 8 Effects of corn silage harvest period, dose of Lactiplantibacillus plantarum and their interaction on the relative abundance of bacteria on the genus level

From: Effects of Lactiplantibacillus plantarum inoculation on the quality and bacterial community of whole-crop corn silage at different harvest stages

Genus	Harvest stage		Dose of p.l² (cfu/g)		Treatment¹				SEM	P value
Genus	Milk stage	Dough stage	0	10⁷	ZM	MM	ZD	MD	SEM	Harvest stage	Dose of p.l²	Harvest stage × dose of p.l²
Lactobacillus	75.97^a	86.67^b	78.77	83.85	74.10^a	77.84^ab	83.45^bc	89.87^c	8.76	< 0.01	0.08	0.63
Weissella	1.28^a	2.51^b	2.24^b	1.55^a	1.21^a	1.34^a	3.26^b	1.76^a	1.00	< 0.01	< 0.01	< 0.01
Klebsiella	1.03	1.40	1.47^b	0.95^a	0.96^a	1.09^a	1.98^b	0.82^a	0.65	0.07	< 0.05	< 0.01
Leuconostoc	0.0.21	0.13	0.23^b	0.11^a	0.27^b	0.16^ab	0.20^b	0.06^a	0.12	0.06	< 0.01	0.78
Enterobacter	0.84^b	0.52^a	0.82^b	0.53^a	0.94^b	0.73^b	0.71^b	0.33^a	0.31	< 0.01	< 0.01	0.39
Gluconobacter	0.92^b	0.25^a	0.93^b	0.24^a	1.43^c	0.40^b	0.42^b	0.09^a	0.56	< 0.01	< 0.01	< 0.01
Lactococcus	0.04^b	0.01^a	0.03	0.03	0.04^b	0.04^b	0.02^a	0.01^a	0.02	< 0.01	0.35	0.78
Sphingomonas	0.44^b	0.11^a	0.25	0.31	0.41^b	0.47^b	0.08^a	0.14^a	0.20	< 0.01	0.20	1.00
Pantoea	0.79^b	0.19^a	0.65^b	0.34^a	1.02^d	0.57^c	0.27^b	0.11^a	0.39	< 0.01	< 0.01	0.07
Aquabacterium	0.67	0.47	0.65	0.48	0.65	0.68	0.65	0.29	0.34	0.15	0.22	0.14
Acetobacter	0.42	1.01	0.52	0.91	0.47	0.37	0.56	1.45	1.13	0.21	0.40	0.30
Asaia	1.13^b	0.07^a	0.99^b	0.21^a	1.87^b	0.39^a	0.11^a	0.03^a	0.94	< 0.01	< 0.01	< 0.01
Acinetobacter	0.27	0.21	0.26	0.21	0.29	0.25	0.23	0.18	0.09	0.09	0.18	0.87
Serratia	0.71^b	0.30^a	0.58	0.43	0.72^c	0.70^c	0.44^b	0.16^a	0.30	< 0.01	0.08	0.12
Stenotrophomonas	0.31^b	0.10^a	0.25	0.17	0.39^b	0.23^ab	0.11^a	0.10^a	0.19	< 0.01	0.21	0.25
Methylobacterium	0.11^b	0.06^a	0.09	0.08	0.10^b	0.11^b	0.08^b	0.04^a	0.04	< 0.01	0.29	0.07
Pediococcus	0.37	0.5	0.36	0.51	0.57	0.16	0.15	0.86	0.65	0.60	0.57	< 0.05
Cedecea	0.66^b	0.13^a	0.46	0.33	0.74^b	0.57^b	0.18^a	0.09^a	0.32	< 0.01	0.10	0.58

^a,b,cValues with different superscripts in the same row within each trial factor in the same row (harvest stage, L. plantarum addition and each treatment group) indicate a significant difference (P < 0.05)
¹ZM: 0 cfu/g of L. plantarum + milk stage; MM: 1 × 10⁷ cfu/g of L. plantarum + milk stage; ZD: 0 cfu/g of L. plantarum + dough stage; MD: 1 × 10⁷ cfu/g of L. plantarum + dough stage. The samples ensiled for 42 d were selected
²L.p: L. plantarum

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