Features | RNA extraction methods | ||
---|---|---|---|
Improved CTAB | PCI | MRIP | |
Surfactant/chaotropic agent | Cetyltrimethyl ammonium bromide | Sodium dodecyl sulfate | Guanidinium thiocyanate |
Phenol content | No | Yes | Yes |
Final RNA precipitation | Ice-cooled isopropanol | Absolute ethanol + incubate in -80 °C freezer | Isopropanol + incubate on ice |
Estimated extraction time inclusive of DNase treatment | 4 h | 27 h | 2.5 h |