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Table 1 General differences between improved CTAB method, PCI method and MRIP method

From: Improved CTAB method for RNA extraction of thick waxy leaf tissues from sago palm (Metroxylon sagu Rottb.)

Features

RNA extraction methods

Improved CTAB

PCI

MRIP

Surfactant/chaotropic agent

Cetyltrimethyl ammonium bromide

Sodium dodecyl sulfate

Guanidinium thiocyanate

Phenol content

No

Yes

Yes

Final RNA precipitation

Ice-cooled isopropanol

Absolute ethanol + incubate in -80 °C freezer

Isopropanol + incubate on ice

Estimated extraction time inclusive of DNase treatment

4 h

27 h

2.5 h