Table 2 General differences of improved CTAB method with Untergasser [42]
Features | Improved CTAB method | Untergasser [42] method |
|---|---|---|
RNA pellet re-solubilization | Gentle mixing | 65 °C in water for 15 min |
DNA removal step | DNase treatment | Did not mention which specifically |
RNA precipitation | Ice-cooled isopropanol | Lithium chloride |
Estimated extraction time inclusive of DNase treatment | 4 h | 5 h |