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Table 3 Sago palm leaf tissue RNA extract quality and quantity assay between improved CTAB, PCI and MRIP methods by Eppendorf Biophotometer Plus

From: Improved CTAB method for RNA extraction of thick waxy leaf tissues from sago palm (Metroxylon sagu Rottb.)

Method

Replicate

A230

A260

A280

A340

260/280

260/230

RNA weight (µg)

Improved CTAB

1

0.316

0.637

0.33

0.001

1.93

2.01

255

2

0.131

0.25

0.13

0

1.93

1.9

100

PCI

1

0.299

0.27

0.161

0.008

1.67

0.9

10.78

2

0.354

0.289

0.181

0.017

1.6

0.82

11.56

MRIP

1

0.941

0.189

0.16

0.044

1.18

0.2

7.56

2

1.073

0.209

0.18

0.054

1.16

0.2

8.38

  1. Improved CTAB method gives the highest purity and RNA yield in mature sago palm leaf RNA extraction