Chemical and Biological Technologies in Agriculture

Table 5 Tukey multiple comparison test of 260/280 ratio and 260/230 ratio data between RNA extraction method.

From: Improved CTAB method for RNA extraction of thick waxy leaf tissues from sago palm (Metroxylon sagu Rottb.)

Multiple comparisons
Tukey HSD
Dependent variable	(I) Method	(J) Method	Mean difference (I-J)	Std. Error	Sig	95% Confidence interval
Dependent variable	(I) Method	(J) Method	Mean difference (I-J)	Std. Error	Sig	Lower bound	Upper bound
260/280	CTAB	MRIP	0.7600^*	0.02972	< .001	0.6358	0.8842
	CTAB	PCI	0.2950^*	0.02972	0.004	0.1708	0.4192
	MRIP	CTAB	− 0.7600^*	0.02972	< .001	− 0.8842	− 0.6358
	MRIP	PCI	− 0.4650^*	0.02972	.001	− 0.5892	− 0.3408
	PCI	CTAB	− 0.2950^*	0.02972	.004	− 0.4192	− 0.1708
	PCI	MRIP	0.4650^*	0.02972	.001	0.3408	0.5892
260/230	CTAB	MRIP	1.7550^*	0.05553	< .001	1.5230	1.9870
	CTAB	PCI	1.0950^*	0.05553	< .001	0.8630	1.3270
	MRIP	CTAB	− 1.7550^*	0.05553	< .001	− 1.9870	− 1.5230
	MRIP	PCI	− 0.6600^*	0.05553	.003	− 0.8920	0.4280
	PCI	CTAB	− 1.0950^*	0.05553	< .001	− 1.3270	0.8630
	PCI	MRIP	0.6600^*	0.05553	.003	0.4280	0.8920

The P-values between all the RNA extraction methods are lower than the α (0.05) value hence the 260/280 ratio and 260/230 ratio data between all the RNA extraction methods are significantly with 95% confidence
Based on observed means
The error term is mean square (error) = 0.003
*The mean difference is significant at the 0.05 level

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