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Chemical and Biological Technologies in Agriculture

Table 7 Mature sago palm leaf tissue (T1, T2, T3, N1, N2, and N3 are biological replicates) RNA extract quality and quantity for RNA sequencing by nanodrop and Agilent 2100 bioanalyzer.

From: Improved CTAB method for RNA extraction of thick waxy leaf tissues from sago palm (Metroxylon sagu Rottb.)

Sample

OD 260/280

OD 260/230

RIN

28 s/18 s

Total mass(μg)

T1

1.91

2.61

6.6

1.4

13.5

T2

1.88

2.23

7.4

2.0

18.7

T3

1.96

2.22

7.5

1.7

37.53

N1

1.99

2.62

7.7

2.0

38.34

N2

1.99

2.38

7.4

1.8

31.45

N3

2.01

2.45

7.5

1.9

35.87

  1. The purity, total RNA amount, RIN and the 28 s/18 s number for all the samples are sufficient for RNA sequencing
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