Skip to main content

Table 2 Organic acid contents, pH and microbial population of ensiled sugarcane tops (n = 3)

From: Effects of pyroligneous acid as silage additive on fermentation quality and bacterial community structure of waste sugarcane tops

Items

Treatments

Ensiling days

SEM

Significant

5

10

20

100

D

T

D*T

Dry matter (g/kg FM)

CK

318

313

312

323

2.34

NS

NS

NS

1%PA

330

312

334

317

2%PA

342

324

325

315

pH

CK

4.72bA

4.51bB

4.27C

4.09D

0.05

**

**

NS

1%PA

4.77bA

4.48bB

4.21C

4.03D

2%PA

4.88aA

4.70aB

4.37C

4.05D

Lactic acid (g/kg DM)

CK

7.13aC

10.9aB

14.8aA

12.2aA

1.00

**

*

NS

1%PA

4.47bB

6.65abB

8.89bA

10.2bA

2%PA

1.71cC

1.75bC

4.69cB

8.33bA

Acetic acid (g/kg DM)

CK

1.47bB

2.00bB

2.34bB

4.01bA

0.23

**

**

NS

1%PA

2.26aB

2.63aB

2.95aB

5.24aA

2%PA

2.58aB

2.89aB

3.33aB

6.13aA

Butyric acid (g/kg DM)

CK

ND

ND

ND

ND

1%PA

ND

ND

ND

ND

2%PA

ND

ND

ND

ND

Lactic acid bacteria (Log10 CFU/g FM)

CK

8.41aA

8.39aA

8.02A

7.40B

0.08

**

**

**

1%PA

8.25aB

8.47aA

8.22B

7.27C

2%PA

7.41bB

7.84bAB

8.17A

7.39C

Molds (Log10 CFU/g FM)

CK

4.14

 < 2.00

 < 2.00

 < 2.00

0.18

NS

NS

NS

1%PA

 < 2.00

 < 2.00

 < 2.00

 < 2.00

2%PA

 < 2.00

 < 2.00

 < 2.00

 < 2.00

Yeasts (Log10 CFU/g FM)

CK

5.21A

4.98B

3.88C

 < 2.00D

0.09

**

NS

**

1%PA

5.03A

5.20A

4.86B

 < 2.00C

2%PA

4.84B

5.10A

4.59B

 < 2.00C

Coliform bacteria (Log10 CFU/g FM)

CK

5.22

3.66

 < 2.00

 < 2.00

1.00

NS

NS

NS

1%PA

 < 2.00

 < 2.00

 < 2.00

 < 2.00

2%PA

 < 2.00

 < 2.00

 < 2.00

 < 2.00

Ammonia-N (g/kg TN)

CK

0.47aC

0.80aB

0.94aB

1.19aA

0.06

**

**

**

1%PA

0.21bD

0.45cC

0.63bB

1.00bA

2%PA

0.12cD

0.18cC

0.30cB

0.67cA

  1. 1Means in the same row (A−D) or column (a−c) followed by different letters differ (P < 0.05)
  2. 2FM fresh matter; DM dry matter, CFU colony forming units, TN total nitrogen, CK control; 1%PA, 1% FM pyroligneous acid added; 2%PA, 2% FM pyroligneous acid added; ND not detected, SEM standard error of means; “—”, default; D, ensiling days effect; T treatments effect; D*T, the interaction effect of treatments and ensiling days
  3. *, P < 0.05; **, P < 0.01; NS no significant effect