Effects of pyroligneous acid as silage additive on fermentation quality and bacterial community structure of waste sugarcane tops

Chemical and Biological Technologies in Agriculture

Table 3 Alpha diversity of bacterial community of ensiled sugarcane tops (n = 3)

Items	Treatments	Ensiling days				SEM	Significant
Items	Treatments	5	10	20	100	SEM	D	T	D^*T
Sobs	CK	1093^aB	1069^B	1163^aA	1168^A	11.1	^**	NS	^*
	1%PA	1113^aB	1094^B	1210^aA	1116^B
	2%PA	1023^b	1165	1096^b	1171
Shannon	CK	6.03^a	5.85	6.19^a	6.47^a	0.08	^**	^*	^*
	1%PA	5.70^b	5.86	5.78^b	5.96^b
	2%PA	4.71^cB	5.57^A	5.38^bA	5.66^cA
Simpson	CK	0.96^a	0.94	0.96^a	0.97^a	< 0.01	^*	^**	^**
	1%PA	0.95^a	0.95	0.94^b	0.95^b
	2%PA	0.88^bB	0.93^A	0.93^bA	0.94^bA
Chao	CK	1622^bC	1649^C	1787^A	1747^B	20.5	^**	NS	NS
	1%PA	1745^aB	1678^B	1944^A	1692^B
	2%PA	1526^b	1756	1770	1730
Ace	CK	1644	1644	1770	1750	15.8	^**	NS	NS
	1%PA	1686	1689	1854	1663
	2%PA	1572	1713	1716	1733
Goods-coverage	CK	0.99	0.99	0.99	0.99	< 0.01	NS	NS	NS
	1%PA	0.99	0.99	0.99	0.99
	2%PA	1.00	0.99	0.99	0.99

¹Means in the same row (^A−C) or column (^a−b) followed by different letters differ (P < 0.05)
²CK control; 1%PA, 1% FM pyroligneous acid added; 2%PA, 2% FM pyroligneous acid added, SEM standard error of means, D ensiling days effect, T treatments effect; D^*T, the interaction effect of treatments and ensiling days
^*, P < 0.05; ^**, P < 0.01; NS no significant effect