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Table 1 Chemical compositions (g/kg of DM, unless otherwise stated) and microbial populations (fresh matter basis) of fresh alfalfa at two growth stages

From: Using PICRUSt2 to explore the functional potential of bacterial community in alfalfa silage harvested at different growth stages

Items

Growth stages

SEM

P value

Initial flowering

Full flowering

Chemical compositions (g/kg DM)

 pH

5.81

5.59

0.054

0.165

 Dry matter (g/kg FW)

219b

238a

2.121

 < 0.001

 Water soluble carbohydrates

47.1b

56.4a

1.425

 < 0.001

 Buffering capacity (mEq/kg DM)

208a

131b

1.136

 < 0.001

 Crude protein

187

190

2.579

0.086

 Neutral detergent fiber

314b

337a

2.336

0.046

 Acid detergent fiber

284b

296a

2.843

0.032

Microbial populations (Log10 cfu/g FW)

 Lactic acid bacteria

3.76b

4.33a

0.143

0.026

 Aerobic bacteria

6.76b

7.39a

0.108

0.038

 Yeasts

3.02b

3.89a

0.112

0.015

 Enterobacteriaceae

6.18b

6.96a

0.130

0.036

  1. Means with different letters in the same row (a−b) differ (P < 0.05)
  2. DM dry matter, FW fresh weight, mEq milligram equivalent, cfu colony-forming units, SEM standard error of means