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Chemical and Biological Technologies in Agriculture

Table 1 PCR system

From: Effects of compound water retention agent on soil nutrients and soil microbial diversity of winter wheat in saline-alkali land

Components

Quantity (μl)

Q5 high-fidelity DNA polymerase

0.25

5* Reaction buffer

5

5* High GC buffer

5

dNTP (10 mM)

2

Template DNA

2

Former primer (10uM)

1

Reverse primer (10uM)

1

ddH2O

8.75

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