Effects of cellulase and xylanase on fermentative profile, bacterial diversity, and in vitro degradation of mixed silage of agro-residue and alfalfa

Chemical and Biological Technologies in Agriculture

Table 5 In-vitro dry matter and NDF degradability, rumen pH, ammonia, gas productions and volatile fatty acid of rumen incubated alfalfa mixed silage prepared with enzyme after 48 h of incubation

Item	Treatment¹				SEM²	P value
Item	C	E	X	EX	SEM²	P value
In vitro dry matter degradability (%)	68.60	72.47	69.86	74.26	0.95	0.07
In vitro NDF degradability (%)	61.60	65.91	62.50	64.18	0.73	0.12
pH	6.08	6.05	6.12	5.75	0.09	0.53
Ammonia nitrogen (mg/dL)	25.85	24.06	25.45	25.43	0.58	0.56
Total VFA (mM)	96.75	101.32	101.81	109.78	2.91	0.56
Acetate (mol/100 mol)	52.47	50.59	52.29	51.50	0.73	0.42
Propionate (mol/100 mol)	23.21	23.10	22.98	22.93	0.53	0.79
Acetate to propionate	2.27	2.20	2.29	2.26	0.08	0.05
Butyrate (mol/100 mol)	14.34	14.62	14.46	14.74	0.10	0.66
iso-Butyrate (mol/100 mol)	2.36	2.66	2.44	2.57	0.08	0.12
Valerate (mol/100 mol)	3.29	4.08	3.60	3.67	0.17	0.39
iso-Valerate (mol/100 mol)	4.33	4.94	4.23	4.60	0.17	0.39
Gas production (mL)	126.22	125.43	121.92	121.67	5.07	0.01