Table 1 qRT‒PCR primer sequences for lifespan-related and signalling pathway-related genes
Gene | Primer sequence (5ʹ–3ʹ) | |
|---|---|---|
RP49 | Forward | CCCTCTTCCAGCCATCGTTC |
Reverse | CCACCGATCCAGACGGAGTA | |
S6K | Forward | CGCAGGACGAGATGATGGA |
Reverse | TGGGATGGGTTGGTTGGT | |
TOR | Forward | CCATCCAGCGGTATGCGGTTATC |
Reverse | TTCAGTGGCACCTTCTTCTTGTCG | |
Keap-1 | Forward | CCAACTTCCTCAAGGAGCAG |
Reverse | CGGCGACAAATATCATCCTT | |
Hep | Forward | GTGGATCTGCATGGAGCTGATGTC |
Reverse | CGTTGACCGTCGCCACTGTG | |
Nrf2 | Forward | GGAGATGACGAGGAGGAGAG |
Reverse | GTGGCATAGGAGGCATTG | |
MTH | Forward | AGCGTATATTAGGAGTGAAGAAGG |
Reverse | CCGTAGGAAGAAGGTGTAAGTC | |