Anticandidal effects and chemical compositions of volatile oils extracted from Origanum syriacum, Clinopodium serpyllifolium subsp. fruticosum and Thymbra capitata from Palestine

Over the past decade, researchers have been exploring the potential therapeutic benefits of volatile oils (VOs) in addressing various disorders, particularly those associated with an increase in fungal infections. This study aimed to analyze the chemical compositions of three different thyme species growing in Palestine using gas chromatography–mass spectroscopy (GC–MS) and explore their antifungal characteristics. The thyme species investigated in this research encompass Origanum syriacum L., Clinopodium serpyllifolium subsp. fruticosum (L.) Bräuchler, and Thymbra capitata (L.) Cav. The VOs of the investigated plants were extracted by hydrodistillation technique equipped with Cleavenger apparatus and characterized by utilizing GC–MS equipment. Moreover, the extracted VOs were evaluated for their antifungal activity using the broth microdilution assay against several clinically isolated Candida species and one ATCC strain. The GC–MS characterization results of O. syriacum VO revealed the presence of 22 components and the abundant molecules were thymol (37.36%), carvacrol (27.71%), γ-terpinene (17.47%), and p-cymene (7.80%), while 19 compounds were characterized in the C. serpyllifolium VO and the major components were p-cymene (37.58%), carvacrol (22.93%), and γ-terpinene (21.91%). In addition, 23 compounds were identified in T. capitata VO and the main components were carvone (59.45%), pulegone (21.59%), menthone (4.24%), and isomenthone (3.71%). According to the antifungal assay results, VO extracted from O. syriacum has the highest activity among all the screened VOs. All the VOs screened in this study exhibit promising antifungal activities for various potential medical applications. Consequently, we strongly advocate for further biological investigations of these oils in the near future.


Introduction
In recent years, there has been a noticeable increase in interest in investigating the medicinal potential of plants, with the majority of these studies focusing on historically utilized plants for food and medicine [1][2][3].These natural remedies offer several advantages, including fewer side effects, cost-effectiveness, and non-toxic properties than synthetic drugs [4].A contemporary treatment method has emerged that combines herbal supplements with traditional chemotherapeutic drugs to increase efficacy while minimizing side effects [5].However, herbal medicine is not subjected to the same regulatory processes as conventional medicine [6][7][8].
This investigation seeks to evaluate the therapeutic potential of chemicals found in the VOs of three Palestinian thyme species: Origanum syriacum L. syriacum is a perennial aromatic herb that is native to western Asia, southern Europe, and the eastern Mediterranean [9].It can be found growing in the open or under cultivation.The leaves, which are commonly used as Arab seasonings, have been incorporated into baked goods, teas, fragrances, and flavors, as well as traditional, complementary, and alternative medicine [10].Clinopodium serpyllifolium subsp.fruticosum (L.) Bräuchler is a profusely aromatic perennial herbaceous plant in Palestine's flora.This plant's leaves and flowers are loaded with potent phytochemicals that have been used to treat a variety of medical conditions for centuries [11].Its medicinal characteristics, such as its antimicrobial potential, have been the subject of several investigations [12].
Thymbra capitata (L.) Cav. is an aromatic medicinal and culinary herb that grows in a variety of Mediterranean regions and possesses significant therapeutic properties that are primarily attributable to its VO constituents [13].The VO of T. capitata is highly valued and economically significant due to its unique biological properties.Previous investigations revealed many potential effects, one of which is antimicrobial activity [14].
Candida is a yeast genus that is one of the most common causes of fungal infections worldwide [15].Candida species are found as endosymbionts in a variety of human body locations, including the urogenital tract, the gastrointestinal tract, and the skin [16].While women are more susceptible to vaginal yeast infections, men can also be affected.Certain factors, such as long-term antibiotic use, put both men and women at risk.Fungal infections are more common in diabetics and people who are immunocompromised [17,18].A number of Candida species are well known in medicine for causing diverse illnesses, with C. albicans, C. parapsilosis, C. tropicalis, and C. glabrata causing the majority of opportunistic infections.These are either superficial or systemic infections of adults and neonates involving the bloodstream, urinary tract, vagina, esophagus, and oral cavity [19][20][21][22].
Numerous studies have explored the antifungal properties of VOs, focusing on their potential to hinder the growth of fungal pathogens [23][24][25].While significant headway has been made in understanding these effects, a noticeable research gap pertains to the distinct chemotypes of VOs from different plant sources and their effectiveness against fungal infections.Moreover, a fresh approach to combat Candida infections has emerged: targeting essential functions crucial for the pathogen's virulence [26].

Plant samples collection, preparation, and extraction of VOs
During the flowering time, O. syriacum, C. serpyllifolium, and T. capitata leaves were gathered from the northern regions of Palestine in May 2021.The plants' samples were recognized by the pharmacognosist, Dr. Nidal Jaradat.A voucher specimen was deposited at the An-Najah National University in the Herbal Products Laboratory and deposed with the codes Pharm-PCT-A1729, Pharm-PCT-1575, and Pharm-PCT-690, respectively) at the same laboratory.
The herbs fresh leaves were dried in the shade at room temperature and relative humidity (25 ± 2 C, 55 ± 3 RH), respectively.The dried parts were pulverized to a fine powder of 60 μm in diameter and stored in airtight containers with adequate labeling for future use.All chemicals and reagents were of analytical grade and were supplied from Sigma-Aldrich (Germany).The VOs were extracted by the hydrodistillation technique [27].Briefly, 100 mg of the dried leaves powder was suspended with 1 L of distilled water, and the VOs were extracted utilizing hydrodistillation with Clevenger apparatus operating at atmospheric pressure for 110 min at 100 °C.The obtained oils were chemically dried utilizing magnesium sulfate and stored at 2 °C until further use.

Gas chromatography/mass spectrometry (GC-MS)
Perkin Elmer Clarus 500 Gas Chromatograph apparatus was utilized for the characterizations of the O. syriacum, C. serpyllifolium, and T. capitata VOs components.This apparatus was connected to the Perkin Elmer Clarus 560 mass spectrometer.The separation was achieved by Perkin Elmer Elite-5-MS fused silica capillary column (film thickness 0.25 µm, 30 m × 0.25 mm).The components were recognized by associating the mass spectra of the components with authentic samples and/or the data from NIST, by interpreting the mass spectral fragmentation pattern of the molecules, and by relating retention indices (RIs) computed relative to a reference mixture of n-alkanes (C 6 -C 30 ) [28].

Antifungal activity
Broth microdilution assay was used in the current study to estimate the antifungal activity of O. syriacum, C. serpyllifolium, and T. capitata VOs against clinically isolated Candida isolates and reference ATCC (90028) Candida albicans.
The VOs were dissolved in DMSO to a concentration of 100 µg/mL.The solution was serially micro-diluted (twofold) 10 times in sterile Brain Heart Infusion (BHI) broth.The dilution process was performed under aseptic conditions in 96-well plates.Wells number eleven contained plant-free BHI broth, which was used as a positive control for microbial growth.Wells number 12 contained plant-free and microbial-free BHI broth, which was used as a negative control for microbial growth.Wells 1-11 were inoculated aseptically with the test microbes.All the inoculated plates were incubated at 35 °C for approximately 48 h.The lowest VOs concentration with no visible microbial growth was considered to be the minimal inhibitory concentration (MIC).Fluconazole was used as a positive control for the antifungal activity [29,30].The VOs' antimicrobial activity was assessed in triplicate.
Comparing our findings to those of Al Hafi et al.2016 [31], the main components of the VO of O. syriacum from Lebanon differ from Palestinian O. syriacum VO.In detail, the Lebanese O. syriacum VO mainly contains carvacrol (79%), whereas the carvacrol consists 27.71% from the Palestinian O. syriacum VO while thymol is the major component with 37.36%.Interestingly, thymol's anticandidal effect has been reported in other studies [24,32,33].This variation in the chemical composition of the compositions of VOs from different populations of O. syriacum highlights the influence of environmental factors such as location and climate on the plant's secondary metabolites.Different dominating components, like carvacrol and thymol, may be responsible for variations in the VO's potential bioactivity and medicinal effects.The monoterpenes category constitutes the predominant compounds in VOs known for their anticandidal properties.Notably, these include p-cymene (found in 40 plants), linalool (in 35 plants), γ-terpinene (in 33 plants), carvacrol (in 31 plants), 1-8-cineole (in 30 plants), α-pinene (in 28 plants), and thymol (in 27 plants).Additionally, the sesquiterpene β-caryophyllene is present in 15 out of 100 plants, which could contribute to the expected antifungal activity based on the resulting chemical composition of various plants [26].Regarding the Palestinian T. capitata, in comparison with those collected from Sicily and Spain reported by Verdeguer et al. [14], it was found that the carvacrol is the major component of T. capitata VO from Sicili (77.02%) and from Spain (77.13%), whereas carvone (59.45%) was identified as major components of the T. capitata VO growing in Palestine.These findings highlight the consistent presence of carvacrol in T. capitata VO and emphasize its significance in anticandidal effect that is also reported by Manohar et al. [25].

Antifungal activity
Due to their toxic nature and increasing resistance to current antifungal drugs, the treatment of Candida infections has become challenging.As a result, there is a pressing need for new antifungal agents and alternative approaches, particularly those derived from natural sources [34].
In recent years, the prevalence of fungal infections has risen, particularly among immunocompromised individuals such as those with HIV infection, those undergoing chemotherapy, and organ or bone marrow transplant recipients.These individuals are susceptible to various forms of fungal infections.Candida infections are extremely common in these individuals, resulting in oral, vaginal, and/or systemic candidiasis [35].Furthermore, Abdalrazeq et al. [36] conducted a study on the Thymbra plant from Palestine, comparing it with the Turkish Thymbra previously analyzed by Baydar et al. [37], the VOs derived from Turkish Thymbra exhibited microbial growth inhibition at concentrations below 1/100 (v/v), whereas the Palestinian Thymbra demonstrated antimicrobial effects at concentrations below 1/800.This observation suggests that the Palestinian Thymbra extract exerts stronger antimicrobial activity than its Turkish counterpart, likely attributed to regional differences between the two plants.
In the present study, the antifungal activity of the screened VOs against Candida species was evaluated using the microbroth dilution assay.The results showed that all the tested VOs exhibited antifungal effects.Notably, the VOs extracted from O. syriacum and C. serpyllifolium have the highest anticandidal activity against the C. albicans ATCC strain.While the T. capitata MIC value was very close to fluconazole.Interestingly, the VO extracted from O. syriacum revealed the highest potential against the clinical strains of C. parapsilosis, C. tropicalis, and C. albicans which is also of higher potential than fluconazole.Moreover, C. serpyllifolium VO showed strong inhibitory effects almost on the growth of all clinical strains.Finally, all tested VOs displayed strong antifungal activity compared to fluconazole, which was used as a positive control for the antifungal assay Table 4.
The observed potent anticandidal effects of the VOs extracted from O. syriacum, C. serpyllifolium, and T. capitata can be attributed to the unique chemical compositions of these plant VOs.In O. syriacum VO, the dominant presence of thymol, carvacrol, γ-terpinene, and p-cymene plays a pivotal role.Thymol and carvacrol, renowned for their antimicrobial properties, likely contribute significantly to the robust antifungal activity.These phenolic compounds are known to disrupt cell membranes and vital cellular processes, leading to Candida species' growth inhibition [38].Similarly, C. serpyllifolium VO's effectiveness can be linked to its abundant p-cymene, carvacrol, and γ-terpinene content, all acknowledged for their anticandidal potential [39,40].These compounds may collaborate synergistically to exert strong inhibitory effects.In T. capitata, the high proportion of oxygenated monoterpenes, particularly carvone and pulegone, underpins the VO's potency.Carvone exhibits antifungal properties through membrane disruption, while pulegone may contribute to this effect and potentially target specific pathways in Candida species [41].The minor compounds in these VOs might also contribute synergistically or independently to the observed activity.Overall, the distinctive chemical profiles of these plant VOs, rich in well-known antimicrobial components, likely underlie their potent anticandidal effects.Further mechanistic studies could shed light on the exact interactions and pathways that contribute to the observed activities.
In summary, the VO extracted from O. syriacum has the highest anticandidal activity among all the screened VOs.However, it is worth noting that previous studies ( Considering the intricate chemical composition of plants' oils and the tendency for biological effects to stem from synergistic interactions among their diverse components, pinpointing the primary active compounds is challenging.The assessment of MIC values on reference strains of C. albicans suggests that certain monoterpenes and their derivatives play a crucial role in plants' oils exhibiting potent antifungal properties.Notably, compounds such as terpinyl acetate, α-terpineol, β-linalool, and γ-terpinene demonstrate robust anti-Candida activity when they constitute major constituents within the oils as in the case of Salvia mirzayanii, Plectranthus caninus Roth, and Thymus willdenowii Boiss [44,45].Conversely, the compounds comprising a significant portion of the plants' oil may not always be directly accountable for their activity.This further complicates the challenge of establishing a direct correlation between the plants' chemical composition and its biological effectiveness.
Extensive research has been dedicated to exploring the antifungal potential of individual acyclic and cyclic monoterpenes [46,47].Among the tested compounds, α-terpineol, terpinen-4-ol, 1,8-cineol, and β-linalool have been documented to exhibit particularly swift fungicidal activity.In contrast, γ-terpinene, α-terpinene, terpinolene, and p-cymene displayed a somewhat slower yet still substantial antifungal effect.This dichotomy implies that the presence of the alcohol functional group holds greater significance than the specific cyclic or acyclic structure, concerning the rapid inhibitory impact on fungal growth.This phenomenon is attributed to the superior water solubility of alcohols within aqueous environments and microbial membranes [48,49].The recognition of plant species exhibiting notable anti-Candida activity could serve as a foundational step for a taxonomic strategy aimed at screening closely related species.This approach holds promise in uncovering valuable compounds within their VOs, mirroring the success observed in the domain of medicinal plants.

Conclusion
The

Table 1
Chemical composition of Origanum syriacum volatile oil by GC/MS

Table 2
Chemical composition of Clinopodium serpyllifolium subsp.fruticosum volatile oil by GC/MS

Table 3
Chemical composition of Thymbra capitata volatile oil by GC/MS

Table 4
Antifungal microbroth dilution assay MIC (µg/mL) values of T. capitata, C. serpyllifolium and O. syriacum VOs GC-MS characterization of O. syriacum VO indicated the presence of 22 components, with thymol, carvacrol, and γ-terpinene being the most prevalent.The most dominant components in C. serpyllifolium VO were p-cymene, carvacrol, and terpinene.Furthermore, 23 chemicals were discovered in T. capitata VO, with carvone and pulegone being the predominant components.The VO compositions were different from those found in other geographic areas.According to the antifungal activity results, all VOs from the three Palestinian Thyme species used in this investigation demonstrated strong action.The VOs isolated from O. syriacum and C. serpyllifolium displayed the strongest antifungal action against all clinical and ATCC Candida strains tested.Furthermore, in vivo, studies are necessary to validate the anticandidal activity of these VOs.

Table 5
Previous investigations examined the antifungal activity of T. capitata, C. serpyllifolium, and O. syriacum VOs against C. glabrata, C. albicans, C. tropicalis, and C. parapsilosis using a microbroth dilution test (MIC values)