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Table 4 Fe recovered (%) from iron–humate complexes formed with HA2, after a first (I) and a second (II)a extraction at different hours of shaking (h), with a solution of the siderophore DFOM and a mixture of four organic acids: citric, oxalic, tartaric and ketoglutaric acids (Mix) at different concentrations

From: Potential alteration of iron–humate complexes by plant root exudates and microbial siderophores

Extractant

h

Concentration

0.01 M

0.001 M

0.0001 M

I

II

I

II

I

II

DFOM

1

1.3 a

1.8 a

1.0 a

0.8 a

0.7 a

0.9 ab

2

3.1 a

2.4 a

2.2 ab

1.1 a

1.1 abc

0.7 a

4

4.0 a

2.7 a

2.6 ab

1.1 a

1.3 bcde

1.2 bcd

16

9.2 a

7.4 a

3.8 bc

3.3 bc

1.8 f

1.7 ef

Mix

1

26.6 b

25.4 b

5.4 cd

6.5 de

1.1 abc

1.5 cdef

2

42.0 c

38.1 c

6.9 de

7.7 e

1.5 cdef

2.3 g

4

53.5 d

37.9 c

7.0 de

10.3 f

1.6 def

2.5 gh

16

66.7 e

23.3 b

15.8 g

15.5 g

2.8 ih

3.1 i

  1. All values are average of three replications. Values followed by the same letter within columns are not significantly different by Tukey’s test at the level of P ≤ 0.05
  2. aFe recovered after the second extraction was calculated on the amount of iron left in the complexes after the first extraction