Statistical optimization of chitinase production by Streptomyces rubiginosus SP24 and efficacy of purified chitinase to control root-knot nematode infection in Vigna radiata under controlled conditions

Chemical and Biological Technologies in Agriculture

Table 5 Chitinase activity from the experimental design for the response surface quadratic model

Std. order	Colloidal chitin	Xylose	Peptone	Dihydrogen potassium phosphate	C.A^a	C.A^b
1	0.1	1.1	1	0.25	2.32	2.13
2	0.55	0.2	1	0.25	2.76	2.49
3	0.55	2	0.6	0.1	2.92	2.82
4	0.55	1.1	0.6	0.25	2.12	2.52
5	0.55	0.2	0.6	0.1	1.56	1.88
6	0.55	1.1	0.6	0.25	2.18	2.52
7	0.55	1.1	0.2	0.4	1.96	2.25
8	1	1.1	1	0.25	3.96	3.78
9	0.1	1.1	0.6	0.1	1.68	1.52
10	1	0.2	0.6	0.25	2.38	2.87
11	0.55	0.2	0.2	0.25	1.28	1.61
12	0.1	1.1	0.6	0.4	1.76	1.86
13	0.1	1.1	0.2	0.25	1.62	1.25
14	0.55	2	1	0.25	3.52	3.43
15	0.55	2	0.6	0.4	2.98	3.16
16	0.55	1.1	0.6	0.25	1.92	2.52
17	0.1	0.2	0.6	0.25	1.38	1.22
18	1	2	0.6	0.25	4.28	3.81
19	0.55	2	0.2	0.25	2.78	2.55
20	1	1.1	0.2	0.25	2.56	2.9
21	0.1	2	0.6	0.25	1.24	2.16
22	1	1.1	0.6	0.1	2.72	3.17
23	0.55	1.1	1	0.4	2.82	3.13
24	0.55	0.2	0.6	0.4	2.72	2.22
25	0.55	1.1	0.2	0.1	2.62	1.91
26	0.55	1.1	0.6	0.25	3.48	2.52
27	0.55	1.1	1	0.1	2.72	2.79
28	0.55	1.1	0.6	0.25	2.82	2.52
29	1	1.1	0.6	0.4	4.02	3.51

C.A^a = actual chitinase activity (U/ml) C.A^b = predicted chitinase activity (U/ml)