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Table 8 Different nematode inoculum volumes affecting mung plant (Vigna radiata) in the pot study

From: Statistical optimization of chitinase production by Streptomyces rubiginosus SP24 and efficacy of purified chitinase to control root-knot nematode infection in Vigna radiata under controlled conditions

Sr. no.

Nematode density

Inoculum volume (ml)

No. of galls/root system

Root-knot index

Control/set-1

0

0

0

0

Set-2

1000

10

5

2

Set-3

1000

20

9

2

  1. Control (2 pots), set-1, 2 and 3 are replicates of three pots, and each set is the mean average of three seedlings/pot
  2. Control—addition of only water (pots 1 and 2)
  3. Set-1 contained only chitinase:organic manure formulation (pots 3, 4 and 5)
  4. Set-2 contained chitinase:organic manure formulation as well as 10 ml of inoculum volume of nematode suspension added near the roots of the plant (pots 6, 7, and 8)
  5. Set-3 contained fenamiphos:organic manure formulation as well as 10 ml of inoculum volume of nematode suspension added near the roots of the plant at the beginning of the experiment (pots 9, 10, and 11)
  6. The number of galls per root system was counted as described by Taylor and Sasser [55], where gall index 1 was on a scale of 0–5: 0 = 0 galls; 1 = 1–2 galls; 2 = 3–10 galls; 3 = 11–30 galls; 4 = 31–100 galls; 5 > 100 galls